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The effect of increasing levels of palm kernel meal containing α-β-mannanase replacing maize to growing-finishing hybrid duck on growth performance, nutrient digestibility, carcass trait, and VFA Sjofjan, O.; Adli, D. N.; Natsir, M. H.; Nuningtyas, Y. F.; Bastomi, I.; Amalia, F. R.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 1 (2021): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.1.29-39

Abstract

In this experiment, we investigated the effect of increasing levels of palm kernel meal containing α-β-mannanase replacing maize to growing-finishing hybrid duck on growth performance, nutrient digestibility, and carcass trait. One hundred and ninety- six hybrid ducks [(Peking x Khaki Campbell)] with 410.52 ± 95.25 g BW) were allotted to 5 dietary treatments with 9 ducks (unsexed) per pen and 4 replicates per treatments. These dietary treatments were: NC (negative control; maize-soybean-meal based diet), PKM25 (maize replacement with PKM α-β-mannanase 25%), PKM50 (maize replacement with PKM α-β-mannanase 50%), PKM75 (maize replacement with PKM α-β-mannanase 75%), PKM100 (maize replacement with PKM α-β-mannanase 100%). There was a curve linear decrease in the growing phase and finisher phase in feed intake as the level of α-β-mannanase palm kernel meal increased in the diet. Curvilinear, the result was presented no significant difference (P>0.05) on the initial body weight, growing and finishing phase body weight. The result of this study showed the used α-β-mannanase in the palm kernel meal presented a significant difference (P < 0.05) on the nutrient digestibility of dry matter and crude protein. Furthermore, Carcass traits showed the reflection in line with the final weight and internal organs were better to compare to control 0.5% using α-β-mannanase in the palm kernel meal. In summary, the increase of the level of palm kernel meal with α-β-mannanase enzyme was had a positive effect on the hybrid ducks and the replacement of palm kernel meal by 25% with the addition of the α-β-mannanase enzyme was able to improve digestibility and reduce abdominal  fat of hybrid ducks. 
The Quality of Cryopreserved Semen of Local Chickens Treated with Ringer’s Lactate-Egg Yolk Extender Supplemented with Glycine and Glucose Junaedi; Isnaini, N.; Natsir, M. H.; Susilawati, T.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.161

Abstract

The aim of this study is to investigate the potential of glucose, glycine, and a combination of glucose and glycine supplemented into Ringer’s lactate extender on the quality of pre- and post-cryopreservation of semen of local chickens. Semen samples were collected from KUB roosters. The research procedures involved formulation of the extender, semen collection, assessment of fresh semen, dilution, packaging, cryopreservation, storage, and subsequent assessment of cryopreserved semen. Parameters assessed in fresh, pre-cryopreservation, and post-thawed semen using computer-assisted semen analysis (CASA), included motility (total motility, progressive, static, and slow motilities), kinematics, spermatozoa morphology, malondialdehyde (MDA) concentration, and scanning electron microscopy (SEM). There were four treatments: T1= control, T2= 50 mM glucose, T3= 60 mM glycine, and T4= a combination of 50 mM glucose and 60 mM glycine. Each treatment was repeated 10 times. The results showed that the addition of 60 mM glycine and the combination of 50 mM glucose and 60 mM glycine effectively preserved total motility, maintained progressive motility, and reduced damage to the bent tail during the equilibration process (prior to cryopreservation). In addition, total motility, progressive motility, DAP, DSL, DCL, VAP, VSL, VCL, STR, and LIN were significantly increased after thawing. This approach also minimized the formation of MDA. Furthermore, observations using SEM showed comparatively more intact acrosomes compared to the other treatments. In conclusion, the addition of 60 mM glycine and a combination of 60 mM glycine and 50 mM glucose to Ringer’s lactate-egg yolk extender proved to be effective in preserving the quality of domestic chicken spermatozoa during semen cryopreservation.