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All Journal Nusantara Science and Technology Proceedings
Suzuki , Takuya
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Nusantara Science and Technology Proceedings

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Different Dietary Protein Sources Affect the Intestinal Tight Junctions in the Colon of Mice Rini , Dina Mustika; Pratiwi , Yunita Satya; Munarko , Hadi; Suzuki , Takuya
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0542

Abstract

This study examined the effects of some dietary protein sources on intestinal barrier function in the colon of mice. 24 female ICR mice were randomly grouped into 4 groups (n=6) fed casein, soybean protein, whey protein concentrate (WPC), and egg white diet for 28 days. In this study, we collected the colon to analyzed the TJs proteins. Cecal contents were also collected to analyze the SCFAs. Mice fed with WPC showed the highest serum LBP concentration. Soybean and casein groups had the highest expression of occludin in the colon of mice. Total SCFAs were not significantly different among the groups. Correlation analysis showed that there is a positive correlation between ZO-1 and ZO-2 with total SCFAs. There is a positive correlation between plasma LBP concentration with claudin-3 expression in the colon of mice. These results confirmed that various dietary protein intake has a significant impact on the regulation of intestinal barrier integrity and SCFAs production might be contributing to the observation.
Co-Authors Dina Mustika Rini Hadi Munarko Yunita Satya Pratiwi