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Different Dietary Protein Sources Affect the Intestinal Tight Junctions in the Colon of Mice Rini , Dina Mustika; Pratiwi , Yunita Satya; Munarko , Hadi; Suzuki , Takuya
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0542

Abstract

This study examined the effects of some dietary protein sources on intestinal barrier function in the colon of mice. 24 female ICR mice were randomly grouped into 4 groups (n=6) fed casein, soybean protein, whey protein concentrate (WPC), and egg white diet for 28 days. In this study, we collected the colon to analyzed the TJs proteins. Cecal contents were also collected to analyze the SCFAs. Mice fed with WPC showed the highest serum LBP concentration. Soybean and casein groups had the highest expression of occludin in the colon of mice. Total SCFAs were not significantly different among the groups. Correlation analysis showed that there is a positive correlation between ZO-1 and ZO-2 with total SCFAs. There is a positive correlation between plasma LBP concentration with claudin-3 expression in the colon of mice. These results confirmed that various dietary protein intake has a significant impact on the regulation of intestinal barrier integrity and SCFAs production might be contributing to the observation.
Borax as a Non-Food Grade Addivite in the Perspective of Foof Safety and Human Resources Formation: A Literature Review Yunita Satya Pratiwi; Dina Mustika Rini; Hadi Munarko; Hadi Prayitno; Ristya Widi Endah Yani; Nadia Shoukat; Kaleem Ahmad
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0518

Abstract

Many foodborne disease cases are still existing in Indonesia and worldwide. Chemicals that act as non-food grade additives is one of the cause. Borax is a chemical which still widely used for food in the community. The purpose of writing this scientific article is to analyze the impact of using borax as a non-food grade additive from the perspective of food safety and human resource formation. This article was based on scientific phenomena that occurred in studies conducted by the author in private, which was then analyzed more deeply based on a study of theories. As mentioned in government law, the use of borax for food additives is prohibited. However, borax is still widely used as food additives in the communities. This phenomenon is a threat to the assurance of food security for individuals and society, which in turn will have an impact on the output quality of human resources needed as the subject of future development. Several studies, investigated the negative effects of borax on the biological function of living things, showed that the use of borax significantly affects food safety in terms of human resources formation. Therefore, it needs both a preventive and curative solution for individuals, groups, and the government in an integrated manner. The use of borax in food is a threat to the assurance of food safety for individuals and society, which in turn will affect the quality of human resources so that a preventive and curative solution is needed.
METABOLOMIC TECHNIQUE IN GREEN TEA RESEARCH: A REVIEW Kurnianto, Muhammad Alfid; Rini, Dina Mustika
International Journal of Eco-Innovation in Science and Engineering (IJEISE) Vol. 5 No. 1 (2024): IJEISE
Publisher : UPN Veteran Jatim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/ijeise.v5i1.128

Abstract

Tea, consumed by two-thirds of the global population, had a production of 5.68 million tons in 2017. The cultivation and processing of tea are crucial variables that have a substantial impact on the final product. As a result, many types of tea, such as black, oolong, and green, are produced, each with its own unique flavors and health advantages. Metabolomic techniques, which employ advanced analytical methods, are employed to characterize chemical composition of tea, thereby enabling the assessment of its quality, origin, and bioactivity. This review focused on the application of these techniques to the green tea research. Analytical methods such as UPLC-MS and 1H NMR, when employed in conjunction with multivariate analysis, have proven effective in correlating green tea quality with its chemical constituents, identifying key metabolites such as EGCG, ECG, and caffeine. These metabolomic techniques permit rapid and accurate assessments, enabling the differentiation between high- and low-quality green tea and enhancing the comprehension of its chemical composition and sensory attributes. In addition, a metabolomic approach was employed, utilizing HPLC-TOF-MS, to differentiate between various types of green tea based on their bioactivities. These methods have the capacity to reveal significant variations in metabolite profiles and bioactivities. The application of multivariate analysis enabled the identification of specific bioactive cultivars, thereby demonstrating the efficacy of the technique in evaluating the health-promoting effects of different tea samples. Thus, metabolomic methods represent an optimal approach for a comprehensive and expeditious investigation of the research related to green tea.
Karakteristik Fisikokimia Teh Kulit Manggis (Garcinia mangostana L.) Setelah Penambahan Kayu Manis (Cinnamomum cassia) dan Cengkeh (Syzygium aromaticum L.): Physicochemical Characteristics of Mangosteen Peel (Garcinia mangostana L.) Tea After Addition of Cinnamon (Cinnamomum cassia) and Clove (Syzygium aromaticum L.) Pratiwi, Yunita Satya; Sanjaya, Yushinta Aristina; Rahmawati, Rahmawati; Rini, Dina Mustika
Jurnal Ilmu Gizi dan Dietetik Vol 2 No 4 (2023)
Publisher : Departemen Gizi Masyarakat, Fakultas Ekologi Manusia, IPB dan PERGIZI PANGAN Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jigd.2023.2.4.301-307

Abstract

Mangosteen (Garcinia mangostana L) contains xanthone compounds that are immunomodulatory, but cause a bitter taste in mangosteen peel tea. Therefore, it is necessary to add certain commodities such as cinnamon and cloves which are also rich in antioxidants. The objective of this study was to analyze the effect of cinnamon and clove addition on the physicochemical characteristics of mangosteen peel tea, and to compare the quality of mangosteen peel, cinnamon, and clove blended tea with SNI. The methods of this study was experimental research with a completely randomized design, statistical analysis using anova test and followed by post hoc tukey. The results of statistical test showed a significant effect at p<0.05. Physicoshemical result that will be compare with SNI Tea showed the total ash content of mangosteen peel tea was 2.86-4.04%, water-soluble ash content 37.05-24.16%, acid insoluble ash content 0.83-0.65%, ash alkalinity content 1.31-1.19, lead (Pb) content 1.53 mg/kg, and no detectable Hg metal content. Overall, there is an effect of cinnamon and clove addition on the physicochemical characteristics of mangosteen peel tea. Only formula M1KC1 (12 g mangosteen peel, 3 g cinnamon, and 3 g cloves) that fulfill the SNI Tea for total ash content, there are no samples that fulfill the SNI Tea standards for water-soluble ash content, all samples fulfill the SNI Tea for acid insoluble ash content, ash alkalinity, mercury (Hg), and lead (Pb).