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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Molekul: Jurnal Ilmiah Kimia
Isti'anah, Ismi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Penapisan senyawa bioaktif antibakteri fungi laut endofit asal Pulau Buton Sulawesi Tenggara: Screening bioactive compounds of marine endophytic fungi as antibacteria from Buton Island, Southeast Sulawesi Isti'anah, Ismi; Tarman, Kustiariyah; Suseno, Sugeng Heri; Nugraha, Roni; Effendi, Irzal
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 27 No. 7 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(7)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i7.50489

Abstract

Fungi laut endofit mengandung senyawa bioaktif alami sehingga memiliki kemampuan sebagai antimikroba, antitumor, antivirus, antikanker, dan antiinflamasi. Tujuan penelitian ini adalah menentukan potensi senyawa bioaktif yang dimiliki oleh fungi laut endofit dan aktivitas antibakterinya dalam menghambat pertumbuhan bakteri patogen Vibrio harveyi. Penelitian dilakukan dengan beberapa tahapan, yaitu isolasi, seleksi, uji antagonis, ekstraksi senyawa aktif, dan uji antibakteri. Isolasi dan seleksi fungi laut endofit dilakukan pada sampel rumput laut dan mangrove yang berasal dari Pulau Buton, Sulawesi Tenggara menggunakan media potato dextrose agar (PDA). Isolat sebanyak 35 diuji antagonis dengan bakteri patogen Vibrio harveyi dan menghasilkan zona bening yang berkisar antara 2-21 mm. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan dipekatkan dengan rotary evaporator. Ekstrak isolat fungi laut Aspergillus terreus (WB 1-2) membentuk zona hambat (inhibisi) tertinggi pada bobot 2 mg/mL sebesar 21,8±0,3 mm. Diameter zona hambat ekstrak Aspergillus terreus tersebut lebih besar dibandingkan dengan kloramfenikol (19 mm) yang digunakan sebagai kontrol positif. Hal ini menunjukkan bahwa fungi laut endofit A. terreus dapat digunakan sebagai antibakteri alami dalam pencegahan patogen di dalam bidang akuakultur.
Isolation and Characterization of Cellulase Enzymes from Marine Endophytic Fungi Tarman, Kustiariyah; Hardiningtyas, Safrina Dyah; Mardiana, Rina; Isti'anah, Ismi; Salamah, Ella
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.9857

Abstract

ABSTRACT. Enzymes called cellulases can break down the β 1,4-glycosidic bonds in cellulose to produce glucose or simple sugars. In this study, marine endophytic fungi that were isolated from the seagrass Enhalus sp. were used to obtain cellulase enzymes, assess activity, and identify the ideal pH and temperature for cellulase enzymes. The research involved a number of steps for the isolation and characterization of cellulase enzymes from Enhalus sp. named EN marine fungi, including preparing the seaweed by soaking it in warm and room-temperature water, extracting the cellulase enzymes by cultivating the marine fungi on production media with pretreated seaweed, counting the total cellulase activity was assessed using the Mandels et al. (1976) technique, and purifying the cellulase enzymes by depositing it with ammonium sulfate at a saturation level of 30-80%. According to the findings, on day 9 with seaweed carbon as a source of warm water immersion, cellulase enzyme isolated from seagrass Enhalus sp. had the greatest crude extract activity of total cellulase enzyme activity (FPAse), amounting to 0.0276 U/mL. The cellulase enzyme precipitated at a 70% saturation level of ammonium sulfate with an FPAse activity of 0.0381 U/mL and a specific activity of 1.85 U/mg. At pH 5 and 50 °C, cellulase enzyme deposition in this investigation was at its best. Keywords: cellulase enzyme, extraction, Enhalus sp., seaweed
Co-Authors Ella Salamah Irzal Effendi Kustiariyah Tarman Rina Mardiana Roni Nugraha safrina dyah hardiningtyas Sugeng Heri Suseno