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All Journal Jurnal Teknologi Laboratorium Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan
Bhatia, Sudhir
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Chemistry Health Professions Medicine & Pharmacology Other

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A simple method for isolation of rest of trypsinized stem cells with magnetic beads Bhatia, Sudhir
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.268

Abstract

Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collected. Magnetic beads are being used around the world to isolate different kinds of cells like CD4, CD8, CD34, and other cells. One of the advantages of magnetic isolation is that they can be used to isolate cells from solutions containing even a low number of targeted cells. We conducted the experiments to see whether all remaining MSC can be obtained from a trypsin-treated flask with magnetic beads isolation. During the magnetic isolation with CD90 specific Magnetic beads, it was found under the microscope that there are huge numbers of cells being attached to beads. The results indicate that there are still a huge number of rest cells in the trypsinized flask but most of the users think that they have isolated all cells, which is not true and they are throwing away valuable stem cells. The magnetic beads can be used to isolate the rest of MSC because for many applications, there is a need of the largest number of stem cells for conducting studies like flow cytometry, molecular testing along with pre-clinical and clinical studies.
Rapid Decline in the Uptake Interests for Different SARS CoV-2 Vaccination Doses Over the Post Vaccination Period in Different European Community Countries Bhatia, Sudhir
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 17 No 2 (2023): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i2.6597

Abstract

During the corona virus pandemic outbreak, there are a number of vaccinations approved. They are distributed and used in human beings worldwide. Aim of this study is to analyze the changes in pattern of uptake mood during different dose courses in EU /EEA population. Therefore, there was use of the following methods: The data from official European community website was collected and analyzed with the software for total number of doses distributed and total number of doses applied. Similarly, cumulative percentage of uptake of vaccine from primary course, first booster, second booster and third booster were compared. Results are showing that there is difference of around 0.5 billion doses between the doses distributed and doses used. The uptake interest decreased from primary dose to third booster dose from 85% to 0% among the countries over the time. The conclusion was found as follows: there are discrepancies between the total number of doses distributed and used. There is very strong loss of uptake interest in the populations in EU/EEA with different dose courses. One must study the reasons for such a strong loss of uptake interest and economic losses caused leading to burden on the tax payers.
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