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All Journal Indian Journal of Forensic Medicine & Toxicology
Zainab K. Hashim
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Public Health

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Epidemiological Study of Cutaneous Leishmaniasis in Wasit Province Abdulsada A. Rahi; Zainab K. Hashim; Ali Mohsin Al-Jamea
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16755

Abstract

The present study was conducted to detect the cutaneous Leishmaniasis from a total of suspected casesthat were collected from dermatology center of AL-Karamah Teaching Hospital in Al-Kut city from 1stOctober 2019 to the end of February 2020. Totally 60 patients were included 36 males and 24 females. Thecollected skin specimens were smeared and stained with Giemsa stain, then examined by microscope underoil immersion lenses. The results of current study appeared that 56(93.3%) were positive amastigotes, Theprevalence of CL were 36 (60%) in males and 24(40%) in females, while the highest infection 36(60%)in rural area and lowest 24(40%) in urban area. The percent of infection with Cutaneous leishmaniasisaccording to type of lesions recorded were equal in dry and wet ulcers type (50 % ) for each one.
Comparison between Microscopic Identification and Nested PCR for Detection of Cutaneous Leishmaniasis at Wasit Province Abdulsada A. Rahi; Zainab K. Hashim; Ali Mohsin Al-Jamea
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16758

Abstract

Cutaneous leishmaniosis in Iraq has 2 forms, zoonotic cutaneous leishmaniasis (ZCL), which is mainlycaused by Leishmania major, and anthroponotic cutaneous leishmaniasis (ACL), which is mainly causedby Leishmania tropica . Twenty skin samples were taken from suspected patients with CL and checked forLeishmania amastigote, during the period from October 2019 to February 2020 in Al-Karamah teachinghospital of Kut city, Iraq. The highest infection 100% using Giemsa- smeared and 75% using Nested PCRmethods. Totally 20 patients aged from (1- <40) years old were included in current study.The prevalenceof CL were in males 9(45) and 11(55) in females and high prevalence in age groups (>20) years old. Thecurrent study 12(60%) were brought on Leishmania major and 4(20%) Leishmania tropica using NestedPCR method . In present study the direct smear could be considered a good test for testing the cutaneousLeishmaniasis but Nested PCR assay was more touchy than parasitological technique in diagnosis ofLeishmania species in skin lesions. L.major is the main species responsible of cutaneous leishmaniasis inareas of Wasit Province.
Co-Authors Abdulsada A. Rahi Ali Mohsin Al-Jamea