Magda A. Ali
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Co-infection between Entamoeba histolytica and Helicobacter Pylori in Patients at Wasit Province Abdulsada A. Rahi; Sima’a H. Mohammed; Magda A. Ali
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16756

Abstract

The current study was conducted at Wasit province, Iraq during the period from 28 October 2020 to28 December 2020. The study included one hundred stool samples taken from questionable patients ofEntamoeba histolytica and Helicobacter pylori. Samples were collected from patient attended to GeneralHospital of Martyr Fairuz at Hay district , Saeed Health center and Hospital of Al-Kut. All samples havebeen checked using direct smear lugol’s iodine stain for Entamoeba histolytica and dipstick for Helicobacterpylori. The result showed that 40 samples (40%) out of 100 patients were gave positive by rapid test cassette;females 23 (23%) positive samples and males 17(17%). While 14 samples (14%)out of 100 samples werepositive for Entamoeba histolytica. The highest infection in females 8(8%) and the lowest in males 6(6%).The result showed 10 samples (10%) out of 100 patients positive for both E. histolytica and H. pylori. Thehighest infection was recorded in females 6(6%) and the lowest in males 4(4%).The highest infection of H.pylori in age group (39 and more) years ; females recorded 6(6%) and malesrecorded 6(6%), while the lowest infection in group (>1) years old ; females recorded 1(1%) and malesrecorded 1(1%). The infection rate of Entamoeba histolytica was highest in age group (1-13) years old ;females was recorded 1(1%) and males was recorded 4(4%) , while the lowest in age group (26-39) years old; females recorded 1(1%) while males without infection. Infection with both E.histolytica and H.pylori wasrecorded the highest in group (39 and more) years old ; females was recorded 3(3%) and males was recorded2(2%) while the group (>1) years old do not recorded infections.
A Comparison of use of Microscopic Examination and PCR for Detection of Entamoeba Histolytica at Wasit Province Magda A. Ali
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16759

Abstract

A microscopic examination of multiple feces samples of 100 suspected patients with Entamoeba histolyticainfection who suffered from diarrhea was attended to Al-Karamah Teaching hospital at Wasit Province fromJanuary 2020 to March 2020. The feces samples were collected into twoParts; first of direct wet film preparation were done for each sample using normal saline 0.9 % for detectingthe motility of trophozoites and Lugol’s iodine 5% for demonstrating structures and cyst. The 2nd part waskept in freeze (-20C) until used in PCR method .The current study recorded that the overall prevalence rate of Entamoeba histolytica 80(80%) by microscopicexamination and 88(88%) by PCR methods. The prevalence of E. histolytica was higher among families wholive in rural areas 44(55%) than those in urban areas 36(45%) and in males 49(61.3%) more than females31(38.7%). The highest infection rate 37(46.3) was recorded in age group (21-40) years old. The presentstudy was aimed to compare between the microscopic examination and PCR methods in the diagnosis of E.histolytica in human clinical samples.We concluded our results that using PCR technique was more accurate and sensitive (88%) than microscopicexamination method (80%).