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All Journal Indian Journal of Forensic Medicine & Toxicology
May. T. flieh
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Public Health

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Isolation and Identification of Bacterial Burn Wound Infection in Iraqi Patient Jenan A. Ghafil; May. T. flieh
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16897

Abstract

Burns are one of the most common and devastating forms of trauma. Patients with serious thermal injuryrequire immediate specialized care in order to minimize morbidity and mortality.In current study, 120samples were collected from 120 patients suffering from contaminated burns. The study was conducted afterobtaining ethical approvals from the ethics committee in the Department of Biology, College of Science,University of Baghdad as well after obtaining the patients ’consent. Samples are collected from patientsafter they have stopped using antibiotics for 48 hours.After the swabs had been cultured on different media,conventional biochemical tests to identify bacterial isolates and antimicrobial sensitivity to the most commonantibiotics were performed by vitek 2 compact .The results showed that the highest percentage of bacterialspecies was Proteus mirabilis (31.1 %). The percentage of isolation of P. aeruginosa was 17.78%. The lowestpercentage of bacterial isolates that isolated from infected wound was found in case of Staphylococcusaureus, Pseudomonas fluorescens, Acinetobacterhaemolyticus, Burkholderiacepacia, Salmonella ser.gallinarum, Sphingomonaspaucimobilis, Comamonas testosterone with 2.2 % for each isolate.
Evaluation the Genotoxicity of PHB Nanoparticle by Micronucleus Assay Jenan A.Ghafil; May. T. Flieh
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16898

Abstract

Determining the toxicity of substances in vivo is one of the most important tests that judge whether ornot they are used in the pharmaceutical field. In the present study, the genetic toxicity of treatment dosesof Polyhydroxybutyrate (PHB), PHB nanoparticles, Cefotaxime and complex of PHB nanoparticlesandcefotaxime was evaluated. The effect of these substances on the number and percentage of white bloodcells (WBCs) in mice was also tested (in vivo). Micronucleus assay was used to assess genotoxicity ofabove materials in vivo, as well as the technique of WBCs chamber was used to estimate the total numberof WBCs in mice administrated with above substances. The differential count of WBCs was measuredby staining the smears with leishman stain. The present study demonstrated that there were no significantdifferences (P>0.05) in the number of micronucleus cells in the mice injected with treatment doses of PHBnanoparticles, Cefotaxime and complex of PHB nanoparticlesand cefotaximewhen compared with miceinjected with normal saline. Similar finding was obtained in terms of counting of total WBCs and differentialcount in mice injected with treatment doses of PHB, PHB nanoparticles, Cefotaxime and complex of PHBnanoparticlesand cefotaxime when compared with WBCs total count and differential count in mice injectedwith normal saline (P>0.05). It can be concluded that there is no toxic effect of treatment doses of PHB, PHBnanoparticles, Cefotaxime and complex of PHB nanoparticlesand cefotaxime on mice.
Co-Authors Jenan A. Ghafil Jenan A.Ghafil