Yasin Kareem Amin
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The Effect of Chemical Weapon Exposureon Semen Fluid Analysis and the Determination of SOD and MDA Levels in Peshmerga against ISIS War Yasin Kareem Amin
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17138

Abstract

The identification of chemical weapons as “weapons of mass destruction” highlights their possible damagingconsequences on many civilians’ health.The effect of chemical weapon agents has been documented tocause reproductive toxicity and have adverse effects on semen, leading to infertility in those exposed tothose chemical agents. This study analysesthe semen fluid and follows up the exposed Peshmerga forcesto the chemical weapon during the ISIS war.A prospective cohort study in which 58 exposed Peshmergain three chemical attacks, compared with the same number of the non-exposed Peshmerga. Semen fluidanalysis was performed for all, following up after one year.The serum level of both MDA and SOD wasmeasured. An individual’s BMI was calculated.The participants’ mean agein this study is (32.5) years in thecase group while (35.5) years in the control group, with a standard deviation of5 years in both groups. Allparticipants are male, and no gender effect be seen. All chronicillnesses have been excluded from the study.The mean body mass index is 22.4, with a standard deviation of about 3.1 kg/m. Hypospermia developed in18% of the exposed group(20% of the total participants), oligospermia (20% overall, only 3% of these in thenon-exposed), and decreased motility in 19% of all participants, but 16 of this 19% where the exposed groupwith significant relative risk results, while morphology not changed inboth groups, also the findings showedthat significant elevation in MDA level and SOD activity. Chemical weapons cansignificantly affect semenfluid analysis and interpretation through quantitativedisturbances in the volume and account of sperm, whilethe percentage of abnormal spermcounts has been increased considerably in peoples exposed to chemicalweapons. However, the morphology of semen fluid sperms has not changed to the same degree, and itsfunctioncannot be estimated. Even when functioning, the genetic errors on future generationsmay needfurther testing.
DNA Marker Screening for High-Risk Non-syndromic Hearing Loss Associated to Gene Mutations Yasin Kareem Amin
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17671

Abstract

Background:Congenital hearing loss is a debilitating disease affecting 1–3 out of 1,000 live births. According to WHO’s associated figures, in both ears, 278 million people globally suffer from moderate to extreme hearing loss. Most hearing-loss individuals live in developing countries. Many deafness and hearing problems cases were documented in our region. Those cases’ exact cause is still unknown, so we performed this study and aimed to screen DNA in high-risk Non-syndromic hearing loss patients in Arbil city. Methods: This researchscreened 132 blood samples from (80 newborns and 52 individuals) at (Hiwa Institute for deaf and mutes); their ages patients from 14 to 22 years old.MTRNR1genes were performed for molecular detection of mutant genes. The mutation gene wasamplified by multiplex tetra primer PCR. Result:G- mito-1555-F1, mito-1555-R1 (O), mito-1555-F2 (I), and mito-1555-R2 (I) hearing loss mutations were not observed in 132 blood samples from both classes and genotyped in MTRNR1. For mtDNA 12S rRNA mt.1555A>G, no mutant alleles were detected in all of the tests, and no false-positives were identified.Using all primers, fifty-two samples were easily separated on 2% agarose gel; two were outer primers, and others are inner primers. Two separate bands were observed with 52 molecular samples (wild type at 254bp and control at 341bp).Of 80 samples, 28 have control bands at 341 bp. We did not find any mutation in our 80 samples. Conclusion: MTRNR1 mutation genes were not present in collected samples in deafness-related mutation. Genetic tests for the deafness gene can better diagnose infant congenital NSHL cases than conventional screening procedures.