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All Journal Jurnal Teknologi Pangan dan Gizi Journal of Tropical Biodiversity and Biotechnology International Journal of Cell and Biomedical Science Indonesian Journal of Pharmacology and Therapy
Rifai, Fauziah Novita Putri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Immunology & microbiology Medicine & Pharmacology

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VARIASI IDENTIFIKASI KUALITATIF FORMALIN PADA IKAN TONGKOL (Euthynnus affinis) DI PASAR TRADISIONAL YOGYAKARTA Rifai, Fauziah Novita Putri; Maliza, Rita
Jurnal Teknologi Pangan dan Gizi Vol 20, No 1 (2021)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jtpg.v20i1.2361

Abstract

Penggunaan pengawet non pangan formalin pada bahan makanan saat ini sudah banyak digunakan. Salah satu cara yang digunakan oleh pedagang ikan dalam proses pengawetan, yaitu dengan menggunakan formalin untuk menghambat terjadinya proses pembusukan dan kemunduran mutu ikan oleh mikroorganisme. Formalin dapat menimbulkan efek jangka pendek dan panjang pada kesehatan. Kandungan formalin pada ikan dapat diidentifikasi dengan uji kualitatif dan uji kuantitatif. Metode uji kualitatif formalin yang memiliki tingkat keakuratan tinggi dan spesifik pada sampel ikan segar saat ini belum dilaporkan. Oleh karena itu dilakukan penelitian yang bertujuan untuk menentukan metode kualitatif formalin yang paling akurat dan sesuai untuk identifikasi formalin pada sampel ikan tongkol. Penelitian ini menggunakan 5 metode uji kualitatif diantaranya asam kromatofat (K10H8O8S2), Tollens, KMnO4 0,1 N, Schiff, dan Fehling pada sampel ikan tongkol yang dijual dibeberapa Pasar Tradisional Yogyakarta. Berdasarkan hasil penelitian, tingkat sensitivitas dari 5 metode uji kualitatif formalin yang digunakan pada 15 sampel ikan tongkol yang paling tinggi adalah metode pereaksi Schiff.
Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D Rifai, Fauziah Novita Putri; Hanifa, Mila; Zulfin, Ummi Maryam; Ikawati, Muthi; Meiyanto, Edy
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88238

Abstract

Pentagamavunone-1 (PGV-1), a curcumin analog, is a promising anticancer candidate for several cancers that have been proven in vitro and in vivo. However, the efficacy of PGV-1 against breast cancer is subject to improvement to achieve a more suitable application. Here we propose hesperitin, a citrus flavonoid, to increase the anticancer potency of PGV-1 in luminal breast cancer cells. We use the T47D cell as the model to investigate the effect of co-administration of PGV-1 and hesperitin on cell cycle block, apoptosis modulation, and senescence phenomena. PGV-1 and hesperitin showed strong and weak cytotoxicity with an IC50 value of 2 µM and 100 µM, respectively. The co-treatment of PGV-1 and hesperitin resulted in strong synergistic effects with combination index (CI) value of ≤ 0.2. This combination caused apoptosis in correlation with cell cycle disruption in G2/M phase at 48 h. In particular, PGV-1 and hesperitin combination increased the incidence of cellular senescence significantly higher than the single treatment. Despite its senescence potentiation, hesperitin did not induce senescence in normal cells. Taken together, hesperitin may increase the anticancer potency of PGV-1 by modulating cell cycle arrest and apoptosis via the senescence mechanism. 
Cooperative Impact of Curcuma longa and Phyllanthus niruri Extracts on Cytotoxicity in HCT-116 Cells Ibrahim, Sugeng; Hidayah, Nurul; Rifai, Fauziah Novita Putri; Ginanto, Dede
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.36

Abstract

Background: The combination of herbal extracts has the potential to enhance cytotoxic effects against cancer cells. This study explores the combined cytotoxic effects of Curcuma longa and Phyllanthus niruri extracts on HCT-116 colorectal cancer cells. Objective: To assess the cytotoxic effects of Curcuma longa and Phyllanthus niruri extracts when used in combination and to determine the most effective ratio for inhibiting HCT-116 cell growth. Methods: HCT-116 cells were treated for 24 hours with varying concentrations of Curcuma longa and Phyllanthus niruri extracts based on the IC50 values of each extract administered individually. The concentrations for Phyllanthus niruri were 164 µg/mL (one part), 82 µg/mL (half part), and 41 µg/mL (quarter part), while for Curcuma longa the concentrations were 47 µg/mL (one part), 24 µg/mL (half part), and 12 µg/mL (quarter part). Cytotoxicity was assessed using the MTT assay. Results: The combination of Phyllanthus niruri and Curcuma longa extracts demonstrated varied cytotoxic effects. The most effective combination was identified as Phyllanthus niruri to Curcuma longa ratio of 1:0.25, resulting in a 13.5% cell viability rate. Interaction studies using the Chou-Talalay method indicated that the combination index (CI) revealed the most synergistic effect at a ratio of 0.25:0.50. Conclusion: The study identifies that the combination of Phyllanthus niruri and Curcuma longa extracts exhibits synergistic cytotoxic effects on HCT-116 cells, with the optimal combination showing significant inhibition of cell growth. These findings support further investigation into the synergistic potential of these extracts for colorectal cancer therapy.
Cytotoxic and Antiproliferative Effects of Gynura divaricata Ethanolic Extract on HCT-116 Colorectal Cancer Cells In Vitro Putri Rifai, Fauziah Novita; Hidayah, Nurul; Sakinah, Feby Nur; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.50

Abstract

Background: Colorectal cancer is a major cause of cancer-related morbidity and mortality worldwide. The limitations of conventional treatments such as chemotherapy and radiotherapy have driven the search for alternative therapies with fewer side effects. Objective: This study aimed to investigate the cytotoxic effects of Gynura divaricata extract on HCT-116 colorectal cancer cells and evaluate its potential as a complementary anticancer agent. Methods: Gynura divaricata was extracted using ethanol through a maceration process. HCT-116 cells were cultured and treated with various concentrations of the extract. Cell viability was measured using the MTT assay, and the IC50 value was calculated through linear regression analysis. Results: Treatment with Gynura divaricata extract resulted in a dose-dependent decrease in cell viability. The IC50 value was determined to be 62.09 µg/mL, indicating significant cytotoxic activity against HCT-116 cells. Conclusion: Gynura divaricata exhibits potential as an alternative or adjunctive therapy for colorectal cancer due to its ability to reduce cancer cell viability.
Effects of Extracellular pH Modulation on HIF-1α, c-Myc, and FOXO1 Expression in Colorectal Cancer Cells Ibrahim, Sugeng; Putri Rifai, Fauziah Novita; Arda, Adzani Gaisani
International Journal of Cell and Biomedical Science Vol 4 No 10 (2025)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v4i10.67

Abstract

Background: The tumor microenvironment (TME) of colorectal cancer (CRC) is characterized by an inverted pH gradient, with acidic extracellular and alkaline intracellular conditions that promote tumor progression and metabolic reprogramming. This altered pH landscape regulates key transcriptional drivers of glycolysis and proliferation, including hypoxia-inducible factor-1 alpha (HIF-1α), c-Myc, and the tumor suppressor Forkhead Box Protein O1 (FOXO1). Understanding how extracellular pH influences these regulators may provide new insights for pH-targeted cancer therapy. Methods: Human colorectal carcinoma HCT116 cells were cultured for 24 hours under six extracellular pH conditions (5.5–9.2). The expression of HIF-1α, c-Myc, and FOXO1 was quantified using quantitative real-time polymerase chain reaction (qPCR), and relative fold changes were analyzed by the 2^-ΔΔCt method. Results: Acidic conditions (pH 5.5–6.7) markedly upregulated HIF-1α and c-Myc while strongly suppressing FOXO1 expression. Conversely, mild alkalinity (pH 8.4) reversed this pattern, reducing HIF-1α and c-Myc while restoring FOXO1 expression, suggesting a transcriptional shift from glycolytic to oxidative metabolism. At higher alkalinity (pH 9.2), the expression of all three genes declined, indicating a threshold beyond which excessive pH elevation becomes detrimental to cellular regulation. Conclusion: Extracellular pH critically modulates metabolic gene expression in CRC cells. Acidic conditions activate glycolytic and oncogenic pathways via HIF-1α and c-Myc, while mild alkalinity suppresses these signals and reinstates tumor-suppressive FOXO1 activity. Controlled alkalinization of the TME may therefore represent a promising adjunctive approach to disrupt tumor metabolism and limit cancer progression.
Phyto therapeutic potential of Andrographis paniculata and Catharanthus roseus extract against colorectal cancer HCT-116 cell line Rifai , Fauziah Novita Putri; Hidayah, Nurul; Prabowo, Adam; Pradani, Lisa Nahdalia
Indonesian Journal of Pharmacology and Therapy Vol 6 No 3 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.24709

Abstract

Colorectal cancer (CRC) is the third most common cancer globally and the second leading cause of cancer-related mortality, with over 1.9 million new cases and 935,000 deaths reported in 2020. Despite therapeutic advances, recurrence, drug resistance, and systemic toxicity remain major challenges. Natural products withantioxidant and cytotoxic activity are increasingly investigated as complementary therapies. Andrographis paniculata (Sambiloto), rich in andrographolide, exerts anticancer effects by inducing apoptosis, inhibiting migration and invasion, and modulating PI3K/Akt and NF-κB signalling pathway. Catharanthus roseus (TapakDara) produces vinca alkaloids, including vincristine and vinblastine, which inhibit microtubule polymerization and are widely used in chemotherapy. Combining these extracts may enhance efficacy and reduce toxicity through synergistic interactions. This in vitro study assessed the cytotoxic and synergistic effectsof A. paniculata extract (APE) and C. roseus extract (CRE) on HCT-116 colorectal cancer cells. Extracts were prepared by ethanol maceration, and cytotoxicity was evaluated using the MTT assay at concentrations ranging from 5 to 100 μg/mL. IC₅₀ values were calculated using linear regression, and the combination index(CI) was determined at 1, 1/2 and 1/4 IC₅₀ to evaluate synergism. APE and CRE exhibited comparable cytotoxicity, with IC₅₀ values of 90 μg/mL and 89.5 μg/mL, respectively. The combination treatment revealed synergistic effects (CI <1) at multiple ratios, particularly at 1/4 IC₅₀ (CI = 0.58), demonstrating enhancedcytotoxicity at reduced concentrations. Both APE and CRE demonstrated significant cytotoxic effects against HCT-116 cells. Their combination produced synergistic interactions, suggesting potential as complementary phytotherapeutic agents for CRC with the benefits of dose reduction and minimized toxicity. Further in vivo and mechanistic studies are warranted.
Co-Authors Adityani, Resanti Arda, Adzani Gaisani Edy Meiyanto Ginanto, Dede Hanifa, Mila Ibrahim, Sugeng Maliza, Rita Muthi Ikawati Nurul Hidayah Prabowo, Adam Pradani, Lisa Nahdalia Sakinah, Feby Nur Zulfin, Ummi Maryam