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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Suhendro, I.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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The genetic diversity of heat shock protein 70 gene at promoter and 5' untranslated region in beef cattle Suhendro, I.; Jakaria, J.; Noor, R.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.136-144

Abstract

This study was aimed to identify genetic diversity in the promoter area and 5' UTR (untranslated region) HSP70 (heat shock protein 70) gene in several beef cattle in Indonesia. A total of 86 blood samples of Bali, Madura, PO (Peranakan Ongole), Limousine, and BB (Belgian Blue) cattle were used in this study. The extracted DNA of all blood samples was then analyzed using the direct sequencing method. The genotyping results showed the presence of five SNP (Single Nucleotide Polymorphism) in the promoter region, namely g.-393T>C, g.-343C>A, g.-202T>C and g.-69T>G and three SNPs at 5' UTR, i.e., g.19A>G, g.45C>T, and g.100_101ins30. The frequency of SNP alleles found in PO, Limousine, and BB cattle was in equilibrium, whereas in Bali and Madura cattle populations, the allele frequency was disequilibrium. In general, the level of diversity of observed alleles ranged from low to high (0.26-1.00), where SNP g.19A>G had the widest variety. It was successfully revealed in this analysis that the SNP g.-69T>G binds to both the NF-Y and CAAT box transcription factor. In addition, the 30 bp (base pair) insertions (g.100_101ins30) that were identified in Bali and Madura cattle have never been reported in previous research studies.
Genetic diversity of coding sequence (CDS) region of HSP70 gene in Bali cattle Bonawati, Y. G. M.; Suhendro, I.; Jakaria, J.; Noor, R. R.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 2 (2025): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.2.103-110

Abstract

This study aimed to investigate the genetic diversity of the Heat Shock Protein 70 (HSP70) gene in the coding sequence (CDS) region and its association with physiological responses in Bali cattle. The samples used in this study consisted of 62 Bali cattle from two different locations in Bali and Se-rading. Physiological data, including respiratory rate, heart rate, rectal temperature, and heat tolerance coefficient (HTC), were collected. The HSP70 gene was amplified using two pairs of primers to target the coding region. SNPs of the HSP70 gene were identified through sequencing. The diversity of SNPs in the coding sequence of the HSP70 gene was determined using the FinchTV 1.4.0 application and version X of the Molecular Evolutionary Genetic Analysis (MEGA) program. In contrast, the association of HSP70 gene SNPs with physiological responses in Bali cattle was evaluated using the GLM method in IBM SPSS Statistics 26. The research discovered nine SNPs within the CDS region of the HSP70 gene, comprising eight synonymous SNPs (c.24C>T, c.31C>T, c.117C>A, c.126G>A, c.324G>A, c.333C>T, c.573G>C, c.1074C>T) and one nonsynonymous SNP (c.1265C>T), which caused an amino acid substitution from threonine (T) to methionine (M). The SNP diversity in the coding sequence of the HSP70 gene showed no significant association (P > 0.05) with physiological responses, including respiratory rate, heart rate, rectal temperature, and heat tolerance coefficient (HTC). The SNPs discovered in the coding region of the HSP70 gene exhibited polymorphism in Bali cattle.
Comparison of Growth Traits of Male Bali Cattle (Bos javanicus) with Different Adult Coat Colors Suhendro, I.; Jakaria, J.; Priyanto, R.; Manalu, W.; Hartono, S.; Noor, R. R.
Tropical Animal Science Journal Vol. 47 No. 1 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.1.25

Abstract

Bali cattle have a distinct coloration that indicates the sexual dimorphism of adult cattle. However, coat color deviations are found in bulls due to either genetic impurity or inbreeding. Furthermore, information is needed to determine whether there is a relationship between coat color and cattle performance. This study aimed to evaluate the potential association between coat color deviation and the growth traits of male Bali cattle. A total of 99 adult male Bali cattle from Livestock Breeding and Forage Centre (BPTU) Denpasar, Bali, were used in this study to assess the possible association between coat color and growth capacity. Animals were grouped according to color patterns into full black (FBL), faded black (FDB), black sorel (BSR), and sorel (SRL). Periodically, growth traits of body weight (BW), wither height (WH), body length (BL), and chest circumference (CC) were measured at birth, weaning, and 365 and 730 days of age. The repeated measurement Anova analysis was used to evaluate the relation between coat color and growth traits over time. This study showed no correlation for both traits in calves due to the absence of color deviation in age. However, it turned out to be a significantly positive correlation (p<0.05) when Bali cattle reached puberty at 365 and 730 days. The black adult bulls (FBL & FDB) were significantly larger in growth traits (p<0.05) than their brown (BSR and SRL) counterparts. The coat color of male adult Bali cattle was associated with their growth traits.
Co-Authors Bonawati, Y. G. M. Jakaria Jakaria R. Priyanto R. R. Noor, R. R. R.R. Noor S. Hartono W. Manalu