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Journal : e-Jurnal Rekayasa dan Teknologi Budidaya Perairan

 1 
Penggunaan Suhu Dan Dosis Propolis Yang Berbeda Terhadap Nisbah Kelamin Ikan Guppy (Poecilia Reticulata) Mulyasih, Dwi; -, Tarsim; Sarida, Munti
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.931 KB)

Abstract

Guppy (Poecilia reticulata) is one of undifferentiated sex species that can direct to produce male during embryogenesis and larvae phase. Temperature is relevant environment factors that affect to gonad differentiation in fish. Propolis is a material from honeybees that has potential to be used in sex reversal to replaced 17α-metiltestosteron. Propolis contains chrysin and galangin with the function inhibited testosteron to estradiol conversion. The aim of this research is to determine the best treatment among different ambient temperature and propolis on different dose. The research was conducted in factorial randomize design, using twelve treatments. The treatments were given to pregnant guppy brood stock for 24 hours that were immersed in interaction of ambient temperature 28; 30 and 32oC with propolis on different dose 0; 25; 75 and 100 µl/l. The result showed that interaction between temperature and propolis showed significant difference in producing male guppy (P<0.05) with high percentage of males equal to  68.72 ± 1.91% in the treatments interaction between temperature 32oC and 25µl/l propolis dose, that has given different significantly with others. The treatments were not stimulate the intersex stocks (P>0.05).
Hormon Testosteron Dan Estadiol 17ß Dalam Plasma Darah Induk Betina Ikan Baung (Mystus Nemurus) Kencana Putra, I Gede Deta; -, Wardiyanto; -, Tarsim
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.628 KB)

Abstract

The research about application of bovine testicular extract on female baung catfish (Mystus nemurus) was done for determining an optimum dosage and application periode of bovine testicular extract. The aim of this research is to increase testosteron and estradiol 17β concentration on blood as gonad developing hormone on female baung catfish broodstock. This  research used randomize blocked design with five treatments and three groups. Dosages of bovine testicular extract which used as treatment were 0; 0,75; 1,5; 2,5 and 4,5 mg/kg feed. The periodes of bovine testicular extract applied were 10; 20 and 30 days. This research used female baung catfish with average body weight of 400-500 g. This research done on July-September 2011 at Balai Benih Ikan Sentral (BBIS) Purbolinggo, East Lampung. Testosterone and estradiol 17β concentration was determined by Radioimmunoassay (RIA) technique at Indonesian Research Institute for Animal Production Ciawi, West Java. The result showed that the application of bovine testicular extract could increase testosteron and estradiol 17β concentration in blood plasma. Dosage 2.5 mg/kg feed could increase testosterone concentration on 278.91 ± 66.76 pg/ml and estradiol 17β concentration on 606.25 ± 187.97 pg/ml. The application periode of Bovine Testicular Extract for 30 days could  increase testosteron and estradiol 17β concentration on 254.38 ± 93.84 pg/ml and 514.55 ± 137.22 pg/ml.
Co-Authors Dwi Mulyasih I Gede Deta Kencana Putra Munti Sarida Wardiyanto -