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E.R. Ekoputri .
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Biochemistry, Genetics & Molecular Biology

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Identifikasi infeksi Koi Herpes Virus (KHV) pada ikan Koi (Cyprinus carpio) dengan metode Polymerase Chain Reaction (PCR), imunositokimia dan imunohistokimia S. Edi .; O. Surfianti .; N. Christy .; R. Wiis .; Laminem .; E.R. Ekoputri .; M. Fathoni .; A. D. Koswara .; Nurhaidin .; U. Yanuhar .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Koi herpes vivus (KHV) disease has been diagnosed in koi and goldfish, and KHV is believed to remain in the body of its host to survive so that goldfish have the potential us a carrier virus. The purpose of this test is to look for another alternative method in identzfiing a particular fish disease Koi Herpes Virus (KHV) that is more simple and practical, rapid, precise and accurate as well as the examination results are expected to match the other examination techniques. Sample testing activities are positively infected with KHV derivedfrom Blitar marked by the characteristics of the most visible of the lesions (injuries) is congestion, bleeding onjns or body, or hemorrhagic on the basis of dorsal fins, pectoral fin, and anal Jins and the operculum, necrosis and nodules (nodule) in the gills white. The result of the testing activities identijcation of KHV by PCR, IHC and ICC were by using PCR samples obtained 7 positive KHV with DNA quality between 1.83 to 1.98 and DNA quantity between 31 7.89 to 492.08 at 290 bp DNA bands. It can be concluded that the alternative examination or identification of infections of Koi Herpes Virus (KHV) in addition to the koifish using PCR, immunohistochemistry, also can be developed using immunocytochemistry method (Streptavidin Biotin) due to simpler procedures, work more practical, lower cost, faster time, precise and accurate target.  
Deteksi penyakit TSV (Taura Syndrome Virus) secara PCR pada udang Vannamei (Litopenaeus vannamei) dengan berbagai ekstraksi, suhu dan waktu penyimpanan O. Surfianti .; N.C. Prihartini .; M. Fathoni .; E.R. Ekoputri .; Laminem .; R. Wilis .; E. Pujiastuti .; Sokhib .; A.D.Koswara .
Hemera Zoa Vol. 2 No. 1 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The shrimp farming industries have been adversely affected by epizootics due to viral pathogens, especially Taura Syndrome Virus (TSV). The TSV has been causative agent of economically disastrous epizootics in Panaeus vannamei, causing mass mortalities of 40%-90% in affected post larval and juvenile population. The current diagnostic and detection methods for TSV included clinical signs, gross pathology, in situ hybridization, and PCR. Three RNA extraction methods, i.e. RNA lysis buffer with Guanidine - HCL, RNA lysis buffer with beta-mercaptoethanol dan phenol-chloroform) were used the RNA lysis buffer with beta-mercaptoethanol, constantly had the highest yield as measured (quality and quantity) using a geneQuant spectrophotometer at period af 3 month in -20° C storeage, except the one extracted by phenol - chloroform extraction had the highest yield quantity at -80°C. Each of 3 (three) extraction methods yielded sufficient RNA for positive result in a RT PCR for TSV at period of 1 month, and 2 months, in both temperature storage (-20°C and -80°C), but at period of 3 months, only the phenol - chloroform extraction give positive result after it was stored at -20°C and -80°c.
Co-Authors A. D. Koswara . A.D.Koswara . E. Pujiastuti . Laminem . M. Fathoni . N. Christy . N.C. Prihartini . Nurhaidin . O. Surfianti . R. Wiis . R. Wilis . S. Edi . Sokhib . U. Yanuhar .