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All Journal Biotropika JURNAL ILMIAH INFORMATIKA
Mike Permata Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology

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SISTEM PAKAR MENDIAGNOSA PENYAKIT OSTEOPOROSIS PADA LANSIA MENGGUNAKAN METODE FORWARD CHAINING BERBASIS WEB Mike Permata Sari; Realize Realize
JURNAL ILMIAH INFORMATIKA Vol 7 No 01 (2019): Jurnal Ilmiah Informatika (JIF)
Publisher : Teknik Informatika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (869.927 KB) | DOI: 10.33884/jif.v7i01.906

Abstract

Osteoporosis is a disease characterized by reduced bone mass and a change in the microarchitecture of bone tissue resulting in decreased bone strength and increased bone fragility and the risk of fractures. The difficulty of handling cases of osteoporosis due to lack of attention to handle osteoporosis, lack of income, so do not have the cost of consulting a doctor or the public do not have spare time to go to consult a doctor about osteoporosis. Therefore, the study was conducted based on the need for tools for people to diagnose osteoporosis. The tool is a web-based expert system using forward chaining method. Based on the test results, the expert system works quite well with the value of the system accuracy reached 83.3%. This expert system in its consultation can be run by answering each question with yes or no, all responses are tailored to the patient's perceived complaints. The output of this system is the name of disease, symptoms, description and solution.
The Use of Purple Eggplant Fruit Calyx Stem (Solanum melongena L.) as an Alternative Source of Lactase-Producing Lactic Acid Bacteria Mike Permata Sari; Radhina, Afifa; Indriani, Narita
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.05

Abstract

Lactose intolerance is a condition where the body cannot digest lactose due to a deficiency of the lactase enzyme in the digestive system, leading to various health issues and indirectly increasing the risk of stunting. To prevent lactose intolerance, supplements containing the lactase enzyme or foods and drinks low in lactose, such as yoghurt, are recommended. Yoghurt production requires microorganisms capable of synthesizing the lactase enzyme, such as lactic acid bacteria (LAB). This research is an experimental study exploring the potential of purple eggplant stalks and petals (Solanum melongena L.) as a new source of LAB capable of synthesizing the lactase enzyme. The experiment began with making yoghurt from milk with added purple eggplant calyx stems as initial proof of LAB presence. Milk was chosen as the initial growth medium because it contains lactose, which acts as a substrate for the lactase enzyme found in LAB. The obtained yoghurt was then inoculated into selective media, resulting in the growth of gram-positive bacilli colonies. The lactase enzyme was isolated from LAB using the sonication method. Optimum enzyme activity was achieved at 70°C and pH 7, measuring 0.0061 U/Mg. This research concludes that purple eggplant stalks and petals contain LAB capable of synthesizing the lactase enzyme. Hopefully, this research will progress to purifying the lactase enzyme and developing lactase enzyme supplements, which can be tested as a therapy for lactose intolerance.
Co-Authors Indriani, Narita Radhina, Afifa Realize, Realize