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Effect of bacteriocin from Lactobacillus sp. Var. SCG 1223 on microbiological quality of fresh meat Usmiati, Sri; ., Miskiyah; R.A.M., Rarah
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Inhibition technology of microorganism on meat can be done biologically and chemically. Biologically, inhibition to microbe can be conducted by addition antimicrobe, for example bacteriocin that have character as biopreservative. The aim of this research was to study the microbiological quality of fresh meat with bacteriocin isolated from Lactobacillus sp. SCG 1223 storaged at certain addition and temperature with different storage time. This experiment was done based on Completely of Random Design (CRD) with factorial arragement 3 x 4 for meat kept at room temperature (270C) and 3x3 for meat kept at cold temperature (4ÂºC) in three replications. The first factor was addition of biopreservative: addition bacteriocin (B), without addition antimicrobia (TB), and addition of nisin (N) on fresh meat. The second factor was different storage time at room temperature (H0, H6, H12 and H18) and low temperature (D0, D14, D28). Other treatments as indicators was contaminated with indicator bacteria (S. thypimurium, E. coli, L. monocytogenes). Variable analyzed were initial TPC (Total Plate Count) and total indicator bacteria S. thypimurium; E. coli; L. monocytogenes at fresh meat, meat quality consist of total indicator of bacteria, pH value of meat; and protein level. Result indicated that bacteriocin from Lactobacillus sp. SCG 1223 isolat could inhibit bacteria growth (S. thypimurium, L. monocytogenes, and E. coli. Bacteriocin produced by Lactobacillus sp. can work at room temperature (270C) and cold temperature (40C). Nisin effectivity almost same to bacteriosin produced from Lactobacillus sp. SCG 1223 isolated from cow fresh milk in inhibiting Gram positive L. monocytogenes. Key Words: Microbiological, Bacteriocin, Lactobacillus sp. Var. SCG 1223

Effect of proteolitic enzymes with probiotic of lactic acid bacteria on characteristics of cow milk dadih ., Miskiyah; Usmiati, S.; ., Mulyorini
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Texture of dadih from cow milk tends to be soft, while dadih from buffalo milk have more compact and solid texture. Enzyme is one of food additives that may produce fermented products made from cow milk that has same charcteristic as dadihâs from buffalo milk. Lactic acid bacteria in fermented milk affect product characteristics. This study aimed to determine the effect of combination of enzyme and probiotic lactic acid bacteria on the characteristics of cows milk dadih. The study was aime designed using completely randomized design (CRD) with 9 treatments, A: renin 2 ppm + 3% Lactobacillus casei; B: renin 2 ppm + 3% B. longum); C: renin 2 ppm + 1.5% L. casei + 1.5% B. longum; D: crude extract of Mucor sp. 0.5 ppm + 3% L. casei; E: crude extract of Mucor sp. 0.5 ppm + 3% Brevibacterium longum; F: crude enzyme extract of Mucor sp. 0.5 ppm + 1.5% L. casei + 1.5% B. longum; G: papain 100 ppm + 3% L. casei); H: papain 100 ppm + 3% B. longum; and F: papain 100 ppm + 1.5% L. casei + 1.5% B. longum). Each treatment was repeated two times. Results showed that combination of renin 2 ppm with 3% of L. casei resulted in the best characteristics of cow milk dadih with viscosity 2278 cP; pH 5.63; titrable acidity 0.56%; moisture 75.03%; protein 6.80%; fat 3.35%; carbohydrate 13.21%; LAB total 6.90 x 1010 cfu/g; it also had a flavor, aroma, texture, and general acceptance that mostly preferred by panelists. Key Words: Dadih, Cow Milk, Enzyme, Lactobacillus casei, Bifidobacterium longum

Effect of proteolitic enzymes with probiotic of lactic acid bacteria on characteristics of cow milk dadih Miskiyah .; S. Usmiati; Mulyorini .
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 4 (2011): DECEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Texture of dadih from cow milk tends to be soft, while dadih from buffalo milk have more compact and solid texture. Enzyme is one of food additives that may produce fermented products made from cow milk that has same charcteristic as dadih’s from buffalo milk. Lactic acid bacteria in fermented milk affect product characteristics. This study aimed to determine the effect of combination of enzyme and probiotic lactic acid bacteria on the characteristics of cow's milk dadih. The study was aime designed using completely randomized design (CRD) with 9 treatments, A: renin 2 ppm + 3% Lactobacillus casei; B: renin 2 ppm + 3% B. longum); C: renin 2 ppm + 1.5% L. casei + 1.5% B. longum; D: crude extract of Mucor sp. 0.5 ppm + 3% L. casei; E: crude extract of Mucor sp. 0.5 ppm + 3% Brevibacterium longum; F: crude enzyme extract of Mucor sp. 0.5 ppm + 1.5% L. casei + 1.5% B. longum; G: papain 100 ppm + 3% L. casei); H: papain 100 ppm + 3% B. longum; and F: papain 100 ppm + 1.5% L. casei + 1.5% B. longum). Each treatment was repeated two times. Results showed that combination of renin 2 ppm with 3% of L. casei resulted in the best characteristics of cow milk dadih with viscosity 2278 cP; pH 5.63; titrable acidity 0.56%; moisture 75.03%; protein 6.80%; fat 3.35%; carbohydrate 13.21%; LAB total 6.90 x 1010 cfu/g; it also had a flavor, aroma, texture, and general acceptance that mostly preferred by panelists. Key Words: Dadih, Cow Milk, Enzyme, Lactobacillus casei, Bifidobacterium longum

Effect of bacteriocin from Lactobacillus sp. Var. SCG 1223 on microbiological quality of fresh meat Sri Usmiati; Miskiyah .; Rarah R.A.M.
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Inhibition technology of microorganism on meat can be done biologically and chemically. Biologically, inhibition to microbe can be conducted by addition antimicrobe, for example bacteriocin that have character as biopreservative. The aim of this research was to study the microbiological quality of fresh meat with bacteriocin isolated from Lactobacillus sp. SCG 1223 storaged at certain addition and temperature with different storage time. This experiment was done based on Completely of Random Design (CRD) with factorial arragement 3 x 4 for meat kept at room temperature (270C) and 3x3 for meat kept at cold temperature (4ºC) in three replications. The first factor was addition of biopreservative: addition bacteriocin (B), without addition antimicrobia (TB), and addition of nisin (N) on fresh meat. The second factor was different storage time at room temperature (H0, H6, H12 and H18) and low temperature (D0, D14, D28). Other treatments as indicators was contaminated with indicator bacteria (S. thypimurium, E. coli, L. monocytogenes). Variable analyzed were initial TPC (Total Plate Count) and total indicator bacteria S. thypimurium; E. coli; L. monocytogenes at fresh meat, meat quality consist of total indicator of bacteria, pH value of meat; and protein level. Result indicated that bacteriocin from Lactobacillus sp. SCG 1223 isolat could inhibit bacteria growth (S. thypimurium, L. monocytogenes, and E. coli. Bacteriocin produced by Lactobacillus sp. can work at room temperature (270C) and cold temperature (40C). Nisin effectivity almost same to bacteriosin produced from Lactobacillus sp. SCG 1223 isolated from cow fresh milk in inhibiting Gram positive L. monocytogenes. Key Words: Microbiological, Bacteriocin, Lactobacillus sp. Var. SCG 1223

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