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Toto Poernomo, Achmad
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Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

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Effect of pH, Temperature, and Metal Activator on The Activity of Fibrinolytic Enzymes Produced by Pseudomonas Aeruginosa Ts 6.4 Hanum, Siti Halima; Toto Poernomo, Achmad; Sudjarwo, Sudjarwo; Rosyidah, Shofiatur
Berkala Ilmiah Kimia Farmasi Vol. 9 No. 1 (2022): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikfar.v9i1.40890

Abstract

Fibrinolytic enzymes were found in several microorganisms, including Pseudomonas aeruginosa TS 6.4, which was isolated from sea cucumbers (Paracaudina australis). Sea cucumbers possessed many pharmacological activities, such as antithrombotic and antihyperlipidemic effects. In addition, fibrinolytic enzymes had been identified in Stichopus japonicus, one of the sea cucumber species. The activity of fibrinolytic enzymes was influenced by pH, temperature, activators, inhibitors, and the concentration of enzyme-substrate. This study aimed to determine the pH and optimum temperature of fibrinolytic enzyme activity of Pseudomonas aeruginosa TS 6.4. The optimum pH was achieved at pH 5-6, while the optimum temperature was reached at 50°C. The effect of the addition of metal activators CaCl2, MnCl2, MgCl2, ZnCl2, and CoCl2 on the activity of fibrinolytic enzymes was also studied. The results showed that the fibrinolytic activity of Pseudomonas aeruginosa TS 6.4 was affected by Ca2+, Mg2+, Mn2+, Zn2+, and Co2+.Keywords: Pseudomonas aeruginosa TS 6.4, Fibrinolytic Enzyme Activity, pH, Temperature, Metal activator
Screening and Identification of The 16S rRNA Sequence Phylogeny of Fibrinolytic Bacteria in Shrimp Paste Sari, Maharani Puspita; Toto Poernomo, Achmad; Prawita, Amiruddin
Berkala Ilmiah Kimia Farmasi Vol. 9 No. 1 (2022): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikfar.v9i1.40899

Abstract

Petis was one of the fermentation products made with marine creatures such as fish or shrimp. Fibrinolytic enzymes in some fermentation foods from marine creatures were discovered. Screening of bacteria that produced fibrinolytic enzymes used six petis samples from different home industries. The isolation of samples was performed using the Skim Milk Agar proteolytic test and fibrin plate test. Identification of the bacteria was performed with a gram staining test and 16S rRNA sequencing method. The sample was diluted with NaCl 0.9% up to 10^-7, then inoculated on the SMA media and incubated at 37ºC for 24 hours. All samples showed proteolytic activity, indicated by a clear zone around the colony. Seventeen proteolytic bacterial isolates were tested for fibrinolytic activity with fibrin plates and then incubated at 37ºC for 24 hours. The seventeen isolates showed positive results for fibrinolytic activity, with the highest fibrinolytic index being P3c. The P3c bacterial isolate was identified using 16S rRNA gene sequencing. The result of the identification revealed that the bacteria with strong fibrinolytic activity was Bacillus flexus.Keywords: Fibrinolytic Enzyme, Petis, Screening, Identification, Bacillus flexus
Co-Authors Hanum, Siti Halima Prawita, Amiruddin Rosyidah, Shofiatur Sari, Maharani Puspita Sudjarwo Sudjarwo