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Yusnawan, Eriyanto
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Agriculture, Biological Sciences & Forestry

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Mikroanalisis Kandungan Senyawa Fenolik Total Ekstrak Biji Kedelai dengan Reagen Folin-Ciocalteu Yusnawan, Eriyanto; Utomo, Joko S
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 1 (2017): April 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.825 KB) | DOI: 10.21082/jpptp.v1n1.2017.p73-81

Abstract

One of the roles of phenolic compounds is as chemical defense system to inhibit the growth ofpathogen infection.Folin-Ciocalteu (FC) reagent is used to estimate total phenolic compounds spectrophotometrically. Microanalysis of the phenolic compounds using FC reagent is still limited.This study, therefore, aimed to develop a rapid method to estimate total phenolic compounds insoybean seed extract in a 96-well microplate. Optimum kinetic reaction was achieved after 90minutes of incubation. Response of 25 – 1.000 µg ml-1 of gallic acid was linear (r = 0.99). Hundredsof samples could be analysed using the microplate 2 format in limited time, such as when workingwith soybean germplasm collection.
Co-Authors Utomo, Joko S