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Antibacterial Activity of Green Sirih (Piper betle L) Extract towards Food Pathogens Suliantari .; B S L Jenie; A Apryiantono
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Green sirih (Piper betle L) extract were prepared using water, athanol and ethyl acetate extractionof the dried material. The extracts were tested for their antibacterial activities against Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillius cereus and Listeria monocytogenes. At concentrations10-50% the extracts effectively inhibited the growth of all tested bacteria as shown by the clear zones which varied from 7 to 24 mm, while the minimum inhibitory concentration (MIC) varied from 0.1 to 1 %. E. coli, S. Typhimumirum and S. aureus were more resistance to the green sirih extracts than other tested bacteria. The result showed that in general, ethanol extraction produced the best extract with strong antibacterial activities against E. coli and S. aureus. Analysis of the extract components with GC-MS revealed that sirih extract contained phenol, chavicol, eugenol, caryophylene, cylene, chalarene and others. Key words: Green Sirih (Piper betle L), bioactive compound, antibacterial.
Yoghurt Sinbiotik Berbasis Probiotik Lokal Dapat Mencegah Diare dan Mengubah Status Hematologi Tikus (SYNBIOTIC YOGHURT BASED ON INDIGENOUS PROBIOTIC: IT’S EFFECT ON DIARRHEA AND HEMATOLOGICAL STATUS IN RATS) Made Astawan; Tutik Wresdiyati; Suliantari .; Yenni MS Nababan
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to observe the effect of functional synbiotics yoghurt (made ofindigenous probiotic + oligofructosaccharides) in rats that were infected with Enteropathogenic Escherichiacoli (EPEC), and to detect its effect on hematological status. A total of 25 male Sprague Dawley rats wereused in this study and divided into five treatment groups: (i) negative-control; (ii) positive-control; (iii)synbiotic yoghurt; (iv) synbiotic yoghurt + EPEC; and (v) conventional prebiotic yoghurt. Yoghurt (109 cfu/mL lactic acid bacteria) was given orally using feeding tube at day one until day 4. At day 8 – day 14animals were infected with EPEC (107 cfu/mL) orally using feeding tube; at day 22 blood samples werecollected for hematology analysis (erythrocytes, hematocrit (Hct), hemoglobulin (Hb), platelets, andleucocytes). Post infection with the EPEC diarrhea was developed in rats both in the control positivegroups and in the synbiotic yoghurt + EPEC group. Animals in the positive-control group had a significantlyhigher thrombocytes and leucocytes counts and Hct compared to that in animals in the other groups(P<0.01). Whereas there were no significant effect on the erythrocytes counts and Hb (P>0.05). The treatmentwith synbiotic yoghurt, in both synbiotic yoghurt only and synbiotic yoghurt + EPEC groups significantlyreduced the platelets and leucocytes counts and Hct of rats