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Sumaryono .
Indonesian Biotechnology Research Institute for Estate Crops
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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MORPHOLOGICAL CHANGES DURING THE DEVELOPMENT OF SOMATIC EMBRYOS OF SAGO (Metroxylon sagu Rottb.) Kasi, Pauline D.; ., Sumaryono
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Development of somatic embryos of sago (Metroxylon saguRottb.) on agar-solidified medium are highly varied producingheterogeneous seedlings. Understanding of this phenomenon mayhelp in improving the cultural procedures and conditions of sagosomatic embryogenesis to obtain uniform seedlings in a largescale. This experiment was conducted at the laboratory for plantcell culture and micropropagation, Indonesian BiotechnologyResearch Institute for Estate Crops from January to March 2006to examine morphological changes i.e. color and developmentstages of sago during their somatic embryo development on anagar-solidified medium. Twenty single globular somatic embryosof sago with specific color (yellowish, greenish, and reddish)were cultured in a Petri dish supplemented with a solid medium.The medium was a micronutrients-modified MS (MMS) withhalf strength of macronutrients containing 0.01 mg l-1 ABA, 2mg l-1 kinetin, 20 g l-1 sucrose, 0.5 g l-1 activated charcoal, and2 g l-1 gelrite. Parameter observed was the percentage ofembryo’s number based on color and developmental stage. Theresult showed that at the end of 6-week culture passage, mostoriginally greenish (80.8%) and reddish (95.8%) embryosremained unchanged in their colors, whereas almost half of theoriginally yellowish embryos turned to greenish and only 30%remained yellowish. At the same time, single globular embryoshave changed gradually into the next developmental stages,although not all of the embryos were germinated. The initialcolor of embryo affected the rate of the developmental stagechanges. Yellowish and greenish globular embryos developedmore rapidly into cotyledon or germinant stages at 58% and55% respectively, in 6 weeks than the reddish ones (41%).Therefore, the yellowish and greenish embryos are the bestsources of material for in vitro mass propagation and syntheticseed production of sago.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture ., Sumaryono; Nur`Aeni, Fitri; Ratnadewi, Diah
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine.When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Co-Authors Diah Ratnadewi Nur`Aeni, Fitri Pauline D. Kasi