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KEKERABATAN PLASMA NUTFAH JAMBU METE BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA Randriani, Enny; Listyati, Dewi; ., Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

Genetic Relationship of Cashew Germplasm Based on Random Amplified Polymorphic DNA. Cashew production in Indonesia stilllow (300-463 kg/ha/year) caused by genetic materials used for the cultivation were not superior variety, therefore, some effort to find superiorvariety with high production must be improved. To support those efforts needs wide variation of plant genetic resources. Nowd ays, characteristic dataof cashew germplasm in BALITTRI based on morphological characters, so needed molecular approach to get genetic characteristic s, geneticrelationship and specific characters such as high production marker, one of some techniques that we can use is RAPD marker. Additionally, RAPDmarker is simple, efficient and accurate. The purpose of this study was to know the genetic variation and relationship among cashew germplasm basedon band pattern of DNA by using RAPD technique. The experiment was conducted at Molecular Biology Laboratory of BB-Biogen, Bogor since Maytill November 2009. Genetic material used were MR 851, PK 36, GG1, Laode Kase, Laode Kapala, JT 27, Arsyad Labone, Wonogiri Merah, A x S,F x M, SM 9, C x M, F x A and BO2 by using 25 primers. The activity consisting germplasm collecting of cashew (14 accessions), followed laboratoryactivities such as: DNA extraction and purification, loading and running of PCR product, RAPD and data analysis. Results shows that 25 primersused are 16 primers shown DNA band pattern, one of them was monomorphism and one specific primer which shown DNA band pattern of cashew,i.e: BO2, SM9 and JT27. Germplasm collection of cashew has wide variation. At 70% coefficient, 14 accessions of cashew were divided to threegroups where first group were content 11 individual (MR 851, PK 36, Laode Kase, GG1, Laode Kapala, A x S, F x A, C x M, Arsyad Labone,Wonogiri Merah, and F x M), while second group were content two individuals (BO2 and SM9). Moreover, in first group itself sh own wide variationamong 11 accessions.
EFEKTIVITAS DAN EFISIENSI TEKNIK ISOLASI DAN PURIFIKASI DNA PADA JAMBU METE ., Syafaruddin; Randriani, Enny; Santoso, Tri Joko
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Effectivity and efficiency of DNA isolation and purification technique on cashew plant. Cashew well known as a potential industrial plant to be expanded due to of kernel price and cashew can also be used as conservation plant. As a potential plant, so needed informationmore detail including the molecular information. There are many techniques to conduct DNA isolation depend on kind of plants or plant tissue thatwill be used. The experiment had been conducted at Molecular Biology Laboratory of Indonesian Center for Agricultural Biotec hnology and GeneticResources Research and Development (BB BIOGEN), Bogor on May-July 2011. Young leaf of cashew used as genetic materials which is took fromexperimental station Cikampek, Indonesian Spice and Industrial Crops Research Institute (BALITTRI). While some chemicals were used as the other material. The activities following step: DNA extraction and purification, measurement of DNA concentration and amplification of DNA. Deletion ofresistor enzyme-polysacharide, especially for perennial plant. DNA isolation can be done by breaking down of cell wall, cell membrane and nuclearmembrane. The aim of this experiment was to find the effectivity and efficiency technique of DNA isolation and purification so can be reducing costand time consuming while working in the laboratory. The results shows that conscientiousness of DNA isolation and purification denotes an importantstep to obtain clean and contaminant free of DNA, so banding pattern will be clear. In this technique did not used polypinilpolypirolidone (PVPP)and mercapto-ethanol such as antioxidant, liquid nitrogen, neither over night storage of leaf extraction before used for purification which is often used for perennial plant. In addional, the results shows that band pattern of DNA was very thick and clear, therefore, this techni que can be used for DNA isolation on cashew.
EFEKTIVITAS DAN EFISIENSI TEKNIK ISOLASI DAN PURIFIKASI DNA PADA JAMBU METE ., Syafaruddin; Randriani, Enny; Santoso, Tri Joko
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Effectivity and efficiency of DNA isolation and purification technique on cashew plant. Cashew well known as a potential industrial plant to be expanded due to of kernel price and cashew can also be used as conservation plant. As a potential plant, so needed informationmore detail including the molecular information. There are many techniques to conduct DNA isolation depend on kind of plants or plant tissue thatwill be used. The experiment had been conducted at Molecular Biology Laboratory of Indonesian Center for Agricultural Biotec hnology and GeneticResources Research and Development (BB BIOGEN), Bogor on May-July 2011. Young leaf of cashew used as genetic materials which is took fromexperimental station Cikampek, Indonesian Spice and Industrial Crops Research Institute (BALITTRI). While some chemicals were used as the other material. The activities following step: DNA extraction and purification, measurement of DNA concentration and amplification of DNA. Deletion ofresistor enzyme-polysacharide, especially for perennial plant. DNA isolation can be done by breaking down of cell wall, cell membrane and nuclearmembrane. The aim of this experiment was to find the effectivity and efficiency technique of DNA isolation and purification so can be reducing costand time consuming while working in the laboratory. The results shows that conscientiousness of DNA isolation and purification denotes an importantstep to obtain clean and contaminant free of DNA, so banding pattern will be clear. In this technique did not used polypinilpolypirolidone (PVPP)and mercapto-ethanol such as antioxidant, liquid nitrogen, neither over night storage of leaf extraction before used for purification which is often used for perennial plant. In addional, the results shows that band pattern of DNA was very thick and clear, therefore, this techni que can be used for DNA isolation on cashew.
KEKERABATAN PLASMA NUTFAH JAMBU METE BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA Randriani, Enny; Listyati, Dewi; ., Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Genetic Relationship of Cashew Germplasm Based on Random Amplified Polymorphic DNA. Cashew production in Indonesia stilllow (300-463 kg/ha/year) caused by genetic materials used for the cultivation were not superior variety, therefore, some effort to find superiorvariety with high production must be improved. To support those efforts needs wide variation of plant genetic resources. Nowd ays, characteristic dataof cashew germplasm in BALITTRI based on morphological characters, so needed molecular approach to get genetic characteristic s, geneticrelationship and specific characters such as high production marker, one of some techniques that we can use is RAPD marker. Additionally, RAPDmarker is simple, efficient and accurate. The purpose of this study was to know the genetic variation and relationship among cashew germplasm basedon band pattern of DNA by using RAPD technique. The experiment was conducted at Molecular Biology Laboratory of BB-Biogen, Bogor since Maytill November 2009. Genetic material used were MR 851, PK 36, GG1, Laode Kase, Laode Kapala, JT 27, Arsyad Labone, Wonogiri Merah, A x S,F x M, SM 9, C x M, F x A and BO2 by using 25 primers. The activity consisting germplasm collecting of cashew (14 accessions), followed laboratoryactivities such as: DNA extraction and purification, loading and running of PCR product, RAPD and data analysis. Results shows that 25 primersused are 16 primers shown DNA band pattern, one of them was monomorphism and one specific primer which shown DNA band pattern of cashew,i.e: BO2, SM9 and JT27. Germplasm collection of cashew has wide variation. At 70% coefficient, 14 accessions of cashew were divided to threegroups where first group were content 11 individual (MR 851, PK 36, Laode Kase, GG1, Laode Kapala, A x S, F x A, C x M, Arsyad Labone,Wonogiri Merah, and F x M), while second group were content two individuals (BO2 and SM9). Moreover, in first group itself sh own wide variationamong 11 accessions.
Co-Authors Dewi Listyati Enny Randriani TRI JOKO SANTOSO