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All Journal PREMISE LAW JURNAL Indonesian Journal of Biotechnology Journal of Natural Sciences and Mathematics Research Journal of Tropical Biodiversity and Biotechnology
Abdul Rahman Siregar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics

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Early Detection and Serotyping of Dengue Viruses Clinical Isolates Using Reverse Transcription Polymerase Chain Reaction (RT-PCR) 2 Primers Abdul Rahman Siregar; Tri Wibawa; Nastiti Wijayanti
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.448 KB) | DOI: 10.22146/ijbiotech.7836

Abstract

Recently several methods for confirming Dengue Virus have been developed involve virus isolation, detection of virus antigen, and nucleic acid using PCR. It has been reported that rapid detection method for confirming DHF by Multiplex RT-PCR had been successfully developed. It was more effective than the other methods with a high sensitivity and specivicity were 100% at the early phase (day 1-3). This study was designed to develop rapid detection and serotyping methods for Dengue Virus using RT-PCR 2 primers (Dcon and preM) with annealing temperature was 57oC. The whole blood samples were collected from suspected dengue fever patients that had been confirmed with NS1 kit from R.S. Persahabatan DKI Jakarta and R.S. Prof. Dr. Sardjito DI Yogyakarta during Februari-August 2009. The PCR products showed that in 12 samples, 100 % were postitive with different pattern among the serotypes especially for DEN1 and DEN2, but not for DEN3 and Den4.  This method was also able to confirm the double infection DEN2-DEN3, but not for the other ones because of the unspecific pattern. From the results, it indicated that the 2 primers can be a promising early detection and serotyping method of Dengue Virus which infected the DHF patients. Key words: Dengue Virus, DHF, early detection, serotyping, RT-PCR 2 primers.
ANALISIS YURIDIS KEWENANGAN OTORITAS JASA KEUANGAN DALAM PENGATURAN DAN PENGAWASAN TERHADAP LEMBAGA PEMBIAYAAN KONSUMEN ABDUL RAHMAN SIREGAR
PREMISE LAW JURNAL Vol 14 (2015): Volume XIV Tahun 2015
Publisher : PREMISE LAW JURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.522 KB)

Abstract

A consumer financial institution is a part of business activities in the industry of financial services and does business activities to finance the provisions of goods based on consumers’ needs as to finance motor vehicles, home equipment, electronic goods and housing. The consumer financial institution in legal institution in Indonesia is arranged in Book III of the Civil Codes on Agreement, Law No.21/2011 on Authority of Financial Service, PMK No.84/PMK.012/206 on Financing Company, Presidential Regulation No.9/2009 on Financing Institution and Regulations of Authority of Financing Service which arrange business  activities of financial institutions. The regulating forms of Authority of Financing Service are the rulings of regulations and policies to business activities to finance consumers and in the form of direct and indirect supervisions of authority of financial service. Breaking the regulations/conditions and the Authority of Financing Service does not fulfill the conditions, the financing company will be given a legal sanction, namely an administrative sanction. Keywords        :                      Authority of Financing Service, Regulating and Supervising, Consumer Financial Institution
Biotransformation of n-butanol to Fruity-Like Bio-Flavour by Indonesian Lactic Acid Bacteria Setiyoningrum, Fitri; Adi, Deddy Triyono Nugroho; Priadi, Gunawan; Wibowo, Des Saputro; Octaviana, Senlie; Afiati, Fifi; Fidriyanto, Rusli; Prasetyo, Doni Dwi; Siregar, Abdul Rahman; Vincentlau, Dharma
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.93537

Abstract

Microbial production of aroma compounds is a promising alternative to extracting plants or chemical synthesis. In our research, the Indonesian lactic acid bacteria (LAB) have been utilised as producing fruity-like bio flavour by biotransformation approach using n-butanol as a precursor. The aims of our research are to identify LAB- secondary metabolites categorised fruity-like bio flavour and investigate the changes of glucose, mannitol, xylose, lactic acid and acetic acid in growth medium after fermentation. Our result research showed that n-butanol could be transformed to several fruity like bio flavour such as ethyl butyrate, butyl acetate, butyl formate, ethyl 2-methylbutanoate, ethyl 3-methylbutanoate, 2-heptanone, butyl propanoate, butyl propanoate, butyl 2-methylbutanoate, butyl isovalerate, butyl pentanoate, and butyl hexanoate. All of LABs consumed above 75% of glucose and only Lactococcus lactis KGB1 consumed all the mannitol on fermentation medium. In addition, Lactococcus lactis KGB1 produced the highest xylose, 11.87 g/L LABs produced. Based on the amount of fruity-like bio flavour compound generated, Lactobacillus fermentum WKS2, Lactobacillus fermentum KGL2, Lactococcus lactis KK4, Lactobacillus fermentum WKS3, Lactococcus lactis KGB1, and Lactobacillus fermentum KGL7 could be considered as agent fruity-like bio flavour by biotransformation approach.
Kombucha origin clustering based on 16S metabarcoding datasets analysis Nugroho, Imam Bagus; Darmawan Ari Nugroho; Abdul Rahman Siregar
Journal of Natural Sciences and Mathematics Research Vol. 11 No. 2 (2025): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.v11i2.23949

Abstract

Consumers of fermented products increasingly demand detailed information on product ingredients, quality, health benefits, and origin. Herein, we have chosen kombucha as a model for a fermented product. This study aims to establish the origin information of kombucha using clustering analysis of 16S metabarcoding datasets. We have downloaded and analysed datasets of kombucha 16S metabarcoding originating from 5 distinct places: Brazil, the United States, the United Kingdom, Turkey, and Thailand. We randomly selected datasets from the collection (n = 32) and analyzed them on the SHAMAN server to develop an initial microbiome profile. We implemented hierarchical agglomerative Clustering and found that Ward's method and the Chao distance produced the best cluster tree, which consistently separates kombucha into five distinct clades, reflecting their origin. We have extended our examination to include more datasets (n=13) to build the final cluster tree (total n = 45). We have also assessed the uncertainty of the final Clustering by pvclust in R. The pvclust cluster tree is comparable in topology to the final cluster tree built using Ward's method and Chao distance. The pvclust cluster tree features stable clades that are highly supported by AU (Approximately Unbiased) values (p-value ≥ 95%). Each kombucha was also placed correctly and consistently according to its respective origin. We have successfully conducted analyses and demonstrated that a simple clustering method, combining Ward's method and the Chao distance, is the most effective for classifying kombucha by origin using a 16S metabarcoding dataset.
Co-Authors Adi, Deddy Triyono Nugroho Fidriyanto, Rusli Fifi Afiati Nastiti Wijayanti Nugroho, Darmawan Ari Nugroho, Imam Bagus Prasetyo, Doni Dwi Priadi, Gunawan Senlie Octaviana Setiyoningrum, Fitri Tri Wibawa Vincentlau, Dharma Wibowo, Des Saputro