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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
S. W. Adkins
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Catatan Penelitian (Research Note) Production of Seedling of Carica papaya L. by Carica parviplora (A.DC) Solms. Interspecific Hybrids Using Embryo Rescue Ernawati, A.; Drew, R. A.; Adkins, S. W.; Godwin, I. D.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 1 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.27 KB) | DOI: 10.24831/jai.v32i1.1435

Abstract

A method of embryo rescue to produce seedlings of Carica papaya x Carica parviflora (A. DC) Solms. interspecific hybrids has been developed. Liquid medium of 0.5 Murashige and Skoog nutrients containing kinetin (0.25 µM) and NAA (0.25 µM) was the best medium to germinate the hybrids and produced the biggest leaf area index of the hybrids. Liquid medium of 0.5 MS nutrients containing kinetin (1.0 µM) and NAA (0.25 µM) produced the highest number of leaves of the hybrids, and produced the longest length of hypocotyl of the hybrids.
INITIATION AND PROLIFERATION OF PAPAYA (Carica papaya L.) SOMATIC EMBRYOS FROM ZYGOTIC TISSUE Ernawati, A.; Adkins, S. W.; Drew, R. A.; Godwin, I. D.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 27 No. 3 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.517 KB) | DOI: 10.24831/jai.v27i3.1572

Abstract

The stufy on the initiation and proliferation of somatic embryos from papaya (C. papaya L.) zygotic embryo explants was undertaken. Best results regarding initiation of embryogenic callus formation was obtained with 90 days after pollination (DAP) zygotic embryos. The medium were liquid and solid MS (0.5) medium containing glutamine (2.7 mM), 2,4-D (9 µM) and sucrose (170 mM) at 6 and 10 weeks after the start of the cultures. In this condition 90% explains produced embryogenic callus formation. The best medium for proliferation of somatic embryos was a liquid MS (0.5) medium containing glutamine (2.7 mM), sucrose (170 mM) in the presence of 2,4-D (9 µM). Using this medium, somatic embryos proliferated three times higher within two weeks.
Co-Authors A. Ernawati I. D. Godwin R. A. Drew