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PEMANFAATAN SERBUK DAUN KETAPANG (Terminalia catappa) UNTUK MENINGKATKAN PERFORMA PERTUMBUHAN DAN SINTASAN BENIH IKAN GABUS (Channa striata) Nugraha, Firman Pra Setia; Murniasih, Siti; Wadjdy, Edy Farid; Sirodiana, Sirodiana; Sudarmaji, Sudarmaji; Rahardjo, Sinung; Saputra, Adang
Media Akuakultur Vol 18, No 2 (2023): Desember, 2023
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.18.2.2023.55-64

Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas penggunaan serbuk daun ketapang dalam memperbaiki media kualitas air benih ikan gabus (Channa striata). Efek dari perbaikan media ini adalah meningkatkan sintasan dan pertumbuhan benih ikan gabus. Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan, yaitu frekuensi pemberian serbuk daun ketapang setiap 3, 5, 7 dan 9 hari sekali. Masingmasing perlakukan dilakukan ulangan sebanyak tiga ulangan. Ikan uji adalah benih ikan gabus dengan rata-rata panjang total dan bobot rata-rata individu berkisar 5,67 ± 0,01 cm3 dan 1,69 ± 0,01 g ekor-1 . Wadah pemeliharaan adalah akuarium kaca berdimensi 70 × 40 × 55 cm dengan volume air 28 L. Sebelum digunakan sebagai perlakuan, daun ketapang dihaluskan hingga mencapai ukuran 42,5 mikron kemudian dimasukan ke dalam kantung teh celup sebanyak 4,67 g. Dosis serbuk daun ketapang yang digunakan yaitu 0,5 g L-1 pada setiap akuarium (6 kantung per akuarium). Selama penelitian, ikan diberikan pakan komersial dengan protein sekitar 40% sebanyak 2 kali sehari secara at satiation. Parameter uji yang diamati antara lain kualitas air, sintasan, pertumbuhan bobot dan panjang mutlak, laju pertumbuhan spesifik, rasio konversi pakan dan retensi protein. Hasil analisis statistik menunjukkan bahwa frekuensi pemberian serbuk daun ketapang setiap 7 hari memberikan tingkat efektifitas terbaik dari segi sintasan mencapai (90,67 ± 2,89%), laju pertumbuhan spesifik (8,92±0,10% hari-1), rasio konversi pakan (0,94 ± 0,01%), rata-rata bobot mutlak (3,57±0,04 g ekor-1), panjang mutlak (2,46±0,06 cm) dan rasio protein (1,05±0,01) pada pemeliharaan benih ikan gabus.The objective of this study was to determine the effectiveness of Terminalia catappa leaf powder on the survival and growth of snakehead juveniles. The study was designed using a completely randomized design. The treatment given was frequency of soaking time for Terminalia catappa leaves powder, namely 3, 5, 7, and 9 days, each treatment consisting of three replications. The test fish were snakehead juveniles with an absolute length and avaradge body weight of 5.67 ± 0.01 cm and 1.69 ± 0.01 g fish-1 which were reared in a glass aquarium with dimensions of 70 × 40 × 55 cm 3 3 with a water volume of 28 L. Before use, the ketapang leaves were mashed until they reach a size of 42.5 microns then put into a 5 g bag. The dose of ketapang leaf powder used was 0.5 g L-1 in each aquarium (6 bag per aquarium). During the research, the fish were given commercial feed with a protein content of ±40% twice daily at satiation. The parameters observed were water quality, survival, absolute weight and length, specific growth rate, feed conversion ratio, and protein retention. The results of statistical analysis showed that the frequency of soaking time for Terminalia catappa leaves powder every 7 days gave the best effectiveness rate of survival (90.67±2.89%), absolute weight growth (3.57±0.04 g fish -1), absolute length (2.46 ± 0.06 cm), specific growth rate (8.92±0.10% day -1) and protein retention (1,05±0,01) of snakehead juvenile rearing.
Susceptibility of three indigenous Indonesian fish species: mahseer (Tor soro), snakehead (Channa striata), and bagrid catfish (Hemibagrus nemurus) against parasites infection Sumiati, Tuti; Taukhid, Taukhid; Andriyanto, Septiyan; Wadjdy, Edy Farid
Journal Omni-Akuatika Vol 18, No 2 (2022): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.2.1000

Abstract

Abstract Mahseer "dewa" (Tor soro), snakehead "gabus" (Channa striata), and bagrid catfish "baung" (Hemibagrus nemurus) are local specific fish species that are promising to be developed as freshwater aquaculture commodities in Indonesia. Disease cases/outbreaks in those fish farming have often been reported, however, there is not much information on the epizootiology and disease status of those fish species. The susceptibility of those fish to parasitic infection was evaluated by natural and artificial infection. Natural infection was conducted by randomly sampling 20 fish at 5-day intervals and lasting for 30 days. In artificial infection, 150 tested fish cohabited with 40 parasite-carrying fish that were known to be definitely infected by the parasite. The observation was carried out by sampling 20 fish at 5-day intervals and lasting for 30 days. The results showed that the three fish species were susceptible to infection with Trichodina spp, Epistylis spp, Tetrahymena spp, Ichtyophthirius multifiliis, Dactylogyrus spp, and Gyrodactylus spp, while the helminth parasite Pallisentis nagpurensis (Acanthocephala) was only identified in snakehead. A number of parasites such as I. multifiliis, Dactylogyrus spp., and Gyrodactylus spp. are likely to be potential obstacles in the cultivation of those fish species, especially in hatcheries and nurseries. Keywords: mahseer, snakehead, bagrid catfish, parasites
PERFORMA VAKSIN IKAN ‘TRIVALEN-SA’ PASCAPENYIMPANAN SELAMA 12 BULAN UNTUK PENCEGAHAN KO-INFEKSI STREPTOCOCCOSIS DAN MOTILE AEROMONAS SEPTICEMIA PADA IKAN NILA (Oreochromis niloticus) Kholidin, Edy Barkat; Wadjdy, Edy Farid; Sudarmaji, Sudarmaji; Sirodiana, Sirodiana; Abduh, Muhammad; Saputra, Adang; Murniasih, Siti; Taukhid, Taukhid
Jurnal Riset Akuakultur Vol 19, No 4 (2024): Desember (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.4.2024.277-298

Abstract

Riset ini bertujuan untuk mengevaluasi efikasi dan keamanan vaksin ikan ‘Trivalen-SA’ pascapenyimpanan selama 12 bulan untuk pencegahan ko-infeksi streptococcosis dan motile Aeromonas septicemia (MAS) pada ikan nila (Oreochromis niloticus). Ikan uji yang digunakan adalah ikan nila yang sudah specific pathogen free dengan bobot 8 ± 1,2 g. Perlakuan yang diberikan adalah aplikasi vaksin melalui: (1) Injeksi satu dosis (0,1 mL per ekor), (2) Injeksi dua dosis (0,2 mL per ekor), (3) Perendaman dalam larutan vaksin pada konsentrasi 107 CFU mL-1,(4) Injeksi 0,1 mL phosphate buffered saline (PBS) sebagai kontrol positif, dan (5) Perendaman dalam air segar selama 30 menit sebagai kontrol negatif.  Efikasi vaksin dievaluasi dengan nilai relative percent survival (RPS) melalui uji tantang terhadap kedua jenis bakteri infektif penyusun formula vaksin. Uji keamanan vaksin dilakukan sesuai metode standar pengujian vaksin ikan. Hasil penelitian menunjukkan bahwa vaksin ikan ‘Trivalen-SA’ pada pengujian ini masih aman dan protektif dengan hasil secara kuantitatif lebih baik karena nilai RPS terhadap infeksi bakteri Aeromonas hydrophila berkisar antara 15,79-29,82%; bakteri Streptococcus agalactiae (non-hemolitik) antara 25,92-48,15%, dan bakteri S. agalactiae (β-hemolitik) antara 18,52-40,75%. Proteksi sinergis terhadap ko-infeksi ketiga jenis atau biotipe bakteri adalah 27,37% untuk injeksi satu dosis; 39,57% untuk injeksi dua dosis, dan 22,54% untuk perendaman. Hasil terbaik berdasarkan kinerja sintasan dan pertambahan bobot tubuh ikan nila dengan nilai RPS mencapai 39,57% apabila diberikan vaksin melalui injeksi dua dosis (0,2 mL per ekor).A study was conducted to determine the efficacy and safety of the ‘Trivalen-SA’ fish vaccine after 12 months of storage in preventing co-infection by streptococcosis and motile Aeromonas septicemia (MAS) in tilapia (Oreochromis niloticus). Specific pathogen-free tilapia with a weight of 8 ± 1,2 g were used as the test fish. The treatments included: (1) Injection with a single dose (0.1 mL per fish), (2) Injection with a double dose (0.2 mL per fish), (3) Immersion with the vaccine solution in 107 CFU mL-1, (4) Injection with 0.1 mL phosphate buffered saline (PBS) as a positive control, and (5) Immersion with freshwater for 30 minutes as a negative control. Vaccine efficacy was assessed using relative percent survival (RPS) values obtained from the challenge tests against each bacterial species in the vaccine. Vaccine safety was evaluated according to standard fish vaccine testing protocols. The results showed that the ‘Trivalen-SA’ vaccine in this study remained safe and effective indicated by RPS value against Aeromonas hydrophila ranged from 15.79 to 29.82%, non-hemolytic Streptococcus agalactiae ranged from 25.92 to 48.15%, and β-hemolytic S. agalactiae ranged from 18.52 to 40.75%. The synergistic protection against co-infection by all three bacterial species or biotypes was 27.37% for the single-dose injection, 39.57% for the double-dose injection, and 22.54% for the immersion method.  The best results based on the survival performance and body weight gain of tilapia with an RPS value reached 39.57% when given the vaccine through double-dose injection (0.2 mL per fish).