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All Journal Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Exact Paper In Compilation (EPIC)
Syahrok, Siti Fatimatus
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Agriculture, Biological Sciences & Forestry Computer Science & IT Decision Sciences, Operations Research & Management Engineering Environmental Science

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Faktor Efektivitas Penggunaan Nematoda Entomopatogen Syahrok, Siti Fatimatus; Suryaminarsih, Penta
Exact Papers in Compilation (EPiC) Vol. 5 No. 1 (2023): Februari 2023
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v5i1.895

Abstract

Pengendalian secara hayati menggunakan nematoda entomopatogen merupakan salah satu upaya dalam pengendalian hama ramah lingkungan serta mampu menjaga ekosistem lingkungan tanaman. Mekanisme patogenesitas NEP melalui bakteri yang berasosiasi dalam fase juvenil infektif sehingga mampu membunuh serangga hama. Keberadaan nematoda entomopatogen dipengaruhi oleh faktor biotik dan abiotik. Faktor biotik meliputi pemilihan jenis spesies, jumlah spesies dan fase hidup. Sedangkan factor abiotik meliputi kondisi lingkungan, kelembaban tanah, suhu tanah, pH tanah, dan kandungan pupuk dan pestisida kimia dalam tanah. Perbanyakan secara konvensional yang efisien tenaga, waktu dan biaya yaitu secara in vivo dengan teknik baiting menggunakan larva Tenebrio molitor. Teknik aplikasi NEP dapat dilakukan dengan berbagai cara penyemprotan dan irigasi, baik menggunakan irigasi tetes, penyemprotan tekanan, penyemprotan kabut dan elektrostatis. Faktor yang perlu diperhatikan mengenai tekanan, jenis nosel dan hama sasaran. Salah satu faktor efektifitas NEP adalah pengembangan formulasi dosis yang efektif. Dosis terbaik pada konsentrasi Heterorhabditis sp. 400 JI/ml tercapai setelah 37,96 jam setelah aplikasi. Pengaplikasian Heterorhabditis sp. pada larva Spodoptera litura menimbulkan mortalitas sebesar 43% setelah 24 jam aplikasi. Pengembangan formulasi dosis harus terus dilakukan dikarenakan setiap spesies NEP memiliki kemampuan patogenesitas berbeda dan tergantung pada hama sasaran.
Resistance of Streptomyces spp. Isolates towards Active Ingredients of the Nematicide Carbofuran and Dazomet, and its Effect on the Mortality of Nematodes Meloidogyne spp. Syahrok, Siti Fatimatus; Suryaminarsih, Penta; Wiyatiningsih, Sri
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.971-978

Abstract

The decrease in tomato plant production is caused by pest and disease attacks, one of which is the nematode Meloidogyne spp. Control measures still rely on synthetic nematicides, which can have negative effects on health, a broad spectrum of impact, microbial diversity, and resistance to attacks by Meloidogyne spp. Therefore, alternative control using biological agents that are resistant to the active ingredients of nematicides (carbofuran and dazomet) and have the ability to inhibit attacks by Meloidogyne spp. is needed. This study aims to test the resistance of Streptomyces spp. isolates to the active ingredients of synthetic nematicides, carbofuran and dazomet, and evaluate their effect on the mortality of Meloidogyne spp. nematodes. The method used is in vitro with the poisoned food technique and nematicidal activity testing against juvenile nematodes. The results show that the TMP isolate has better resistance than SP against carbofuran, with the second highest colony count of 11.47 × 10⁶ CFU/ml. Meanwhile, dazomet significantly inhibited the growth of Streptomyces spp. colonies. The mortality test showed that the TMP isolate was able to achieve a mortality rate of 59.01% in Meloidogyne spp. This proves the potential of Streptomyces spp. as a biological agent in nematode control and its resistance to nematicide residue active ingredients, making it an environmentally friendly alternative in agricultural practices. Keywords: Biological agent, Carbofuran, Dazomet, Meloidogyne spp., Streptomyces spp.
Co-Authors Penta SURYAMINARSIH Sri Wiyatiningsih