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Agustinus Joko Nugroho
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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Purification and Characterization of Streptomyces sp. IK Chitinase Sebastian Margino; Agustinus Joko Nugroho; Widya Asmara
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.573 KB) | DOI: 10.22146/ijbiotech.7820

Abstract

Streptomyces sp. IK isolated from compost inoculants, could produce extra cellular chitinase in a medium containing 0.2% (w/v) colloidal chitin, fermented for 96 hours at 30oC. The enzyme was purified by a combination of ammonium sulphate precipitation and DEAE-Cellulose anion-exchange chromatography. On SDS-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a mass of 71 kDa. Chitinase was optimally active at pH of 6.7 and at 37oC. Km value and Vmax of the protein for colloidal chitin were 2.92 mg/ml and 4.26 ìg/h, respectively.Key words : chitinase, Streptomyces, purification, characterization
Co-Authors Sebastian Margino Widya Asmara