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Warsidah, W.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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Total Phenolic Content, Antioxidant and Antibacterial Activities of Sargassum polycystum of Ethanol Extract from Waters of Kabung Island Safitri, Ikha; Warsidah, W.; Sofiana, Mega Sari Juane; Kushadiwijayanto, Arie A.; Sumarni, T. Novi
BERKALA SAINSTEK Vol 9 No 3 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i3.27199

Abstract

Sargassum policystum is one of the genus Sargassum from the brown macroalgae group which has an abundance of chemical constituents of fucoidan, fucoxanthin, phenolic acids and alginate which have important biological activities, such as antioxidants and antibacterials. This species thrives in the waters of Kabung Island, Bengkayang, West Kalimantan and has not been exploited so that it tends to act as a marine weed. This study aimed to determine the total phenolic content of the ethanolic extract of S. policystum and its antioxidant and antibacterial activity. Determination of the total content of phenolic compounds using the Folin Ciocalteu method, antioxidant testing using the DPPH method (2,2-diphenyl-1-picrylhydrazyl) and the indicator of sample color change with the addition of these radicals was measured by UV-Vis spectrophotometer. Antibacterial testing was carried out by the test sample diffusion method and the inhibition of the growth of the test bacteria Staphylococcus aureus and Escherichia coli as test bacteria was indicated by the formation of a clear area around the sample wells. The results showed that the phenol content of the ethanolic extract of S. policystum was 79.8 mg/g extract, its antioxidant activity (IC50) was 98.903 ppm, classified as a strong antioxidant, while the comparison antioxidant, Vitamin C, had an IC50 of 6.26 ppm. The inhibitory ability of the ethanol extract against the test bacteria used was indicated by the formation of the largest inhibition diameter on S. aureus of 12.4 mm and E. coli of 12.0 mm.
Decomposition Rate of Avicennia lanata in Bakau Kecil Mangrove Forest Area, Mempawah District Ashari, Asri Mulya; Warsidah, W.
BERKALA SAINSTEK Vol 9 No 4 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i4.27198

Abstract

Avicenna lanata is a type of mangrove that dominates the Mempawah Kecil Mangrove Forest. As many as 10 bacterial genera were identified from station 1 which is close to the influence of sea water, and 8 bacterial genera from station 2 which are close to residential areas. This study aims to determine the rate of decomposition of A. lanata leaf litter with 4 observation time periods with an interval of 15 days, namely the 15th, 30th, 45th and 60th days. The observations show that the rate of decomposition of the litter on the 15th day of observation is higher. Namely 0.206 gram/day at station I and 0.19 gram/day at station II, while the lowest rate of decomposition of litter occurred on the 60th day of observation, namely 0.112 gram/day at station I and 0.106 gram/day at station II. In addition, the parameters of physical and chemical of the environment such as DO, temperature, pH and salinity are in the range of their optimum values.
Co-Authors Ashari, Asri Mulya Ikha Safitri Kushadiwijayanto, Arie A. Mega Sari Juane Sofiana Sumarni, T. Novi