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All Journal Makara Journal of Science Madani: Multidisciplinary Scientific Journal
Anwar, Nadiem
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PENINGKATAN UNJUK KERJA HIDROLISIS ENZIMATIK JERAMI PADI MENGGUNAKAN CAMPURAN SELULASE KASAR DARI Trichoderma reesei DAN Aspergillus niger Anwar, Nadiem; Widjaja, Arief; Winardi, Sugeng
Makara Journal of Science Vol. 14, No. 2
Publisher : UI Scholars Hub

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Increasing the Performance of Enzymatic Hydrolysis of Rice Straw Using Mixed Crude Cellulases from Trichoderma reesei and Aspergillus niger. The objective of this work is to compare the effectiveness of mixed crude enzyme cellulase from T. reesei and A. niger with commercial enzyme from A. niger, and to investigate effect of enzyme to substrate ratio to performance of enzymatic hydrolysis of rice straw. The commercial enzyme from Fluka Biochemica was used, and crude enzyme were prepared by solid fermentation with simple media. Before hydrolized, the rice straw was grinded and sieved and then heated at 85 o C with 2% sodium hydroxide for six hours. Hydrolysis was conducted in 300 mL beaker flask equipped with mechanical stirrer. Samples were analyzed by dinitrosalicylic acid method and measured by spectrophotometer. Both of commercial and mixed crude enzyme show that, the higher enzyme to substrate ratio was higher the glucose concentration obtained. However, ratio of glucose obtained to enzyme used become smaller. The mixture of crude enzyme from T. reesesi dan A. niger that produced in this work was two fold more effective to hydrolyze rice straw than using cellulase enzyme of A. niger from Fluka Biochemika
Penggunaan Pelarut Acidified Water dalam Perolehan Antosianin dari Kulit Buah Naga Merah menggunakan Microwave Assisted Extraction (MAE) Shiddiqi, Qifni Yasa Ash; Putri, Tiara Ariani; Hizbiyati, Andini; Prabowo, Bambang Hari; Anwar, Nadiem; Nurramdhania, Anisya; Apriliani, Fatiha
Madani: Jurnal Ilmiah Multidisiplin Vol 3, No 6 (2025): July 2025
Publisher : Penerbit Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.16739241

Abstract

Dragon fruit peels are agricultural waste products that weigh 30-35% of the fruit. Dragon fruit peels contain anthocyanin natural dyes which are quite high. Anthocyanins are a class of flavonoid compounds which are widely divided into plant polyphenols. Anthocyanins are stable at pH 3.5 and temperature 50. The purpose of this study is to determine the anthocyanin content of dragon fruit peels and also to know the quality of natural dyes produced from anthocyanin dragon fruit peels. Intake of anthocyanin dyes is carried out by the extraction method followed by a distillation process so that the anthocyanin extract becomes more concentrated and can be applied directly as a natural dye. The extraction technique used is Microwave Assisted Extraction (MAE) with acidified water solvent. The acid used is citric acid 10% with a ratio of aquades: citric acid 10% is 3:1 and 8:1 with extraction time of 1,3,5 minutes at 350 watts of power. The content of anthocyanin was analyzed using a UV-VIS spectrophotometer. The best results of the content of anthocyanin were at the extraction time of 5 minutes with a solvent ratio of 3: 1 with a concentration of 36.40 mg /100 g.
Co-Authors Apriliani, Fatiha Hizbiyati, Andini Nurramdhania, Anisya Prabowo, Bambang Hari Putri, Tiara Ariani Shiddiqi, Qifni Yasa Ash Sjarief Widjaja Sugeng Winardi