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All Journal Jurnal Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan
Ferry Ardhiansyah
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Health Professions Nursing Public Health Veterinary

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Konsentrasi Serum Anjing yang Optimum untuk Menumbuhkan dan Memelihara Babesia canis dalam Biakan Tutuk Astyawati; Retno Wulansari; Cahyono -; Ferry Ardhiansyah; Ari Rumekso; Dhetty -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.485 KB)

Abstract

The use of cultivation system in vitro is very important in the future study of Babesia canis. Theaim of this study was to cultivate B. canis in vitro using RPMI media with different concentration of dogsera. B. canis infected erythrocytes were collected from splenectomized infected dog. Parasites werecultivated with RPMI 1640 medium supplemented with normal dog sera at the concentration of 10%, 20%and 40%, the culture were then incubated in 5% CO2 , 37oC temperature for 17 days and subcultured every48 hours. The Percentage of Parasitized Erythrocytes (PPE) in culture with 10% dog serum was significantlylower than those 20%, and 40% The used of 20% and 40 % sera were better than 10%. It is recommendedthat 40 % serum can be used for initiation phase of cultivation, whereas 20% concentration were used formaintenance of the culture.
FAKTOR YANG MEMPENGARUHI KLB CAMPAK ANAK USIA SEKOLAH DASAR Ardhiansyah, Ferry; Budhi R, Kamilah; Suwondo, Ari
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 8, No 1 (2020): EDITION MARCH 2020
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.788 KB) | DOI: 10.33366/jc.v8i1.1202

Abstract

Measles is a very contagious disease and still a health problem in Indonesia. Immunization coverage of measles BIAS in Pesawaran District is quite good (98.8%), but the incidence measles outbreaks in elementary school-age children still occur, this can be caused by various child factors, maternal and environmental factors. The purpose to examine the factors that influence outbreaks of measles are children of primary school age. The observational analytics study design with a mix methods approach. The participants consist of 27 cases and 54 controls, in-depth interviews with UKS teachers. Dependent variable was measles outbreak incidence in primary school age, independent variables were contact history, maternal age, maternal education, occupancy density, and UKS teacher knowledge. Bivariate using Chi Square test and multivariate analysis using logistic regression test. The result of bivariate analysis showed that there was a correlation between contact history (p=0,001), maternal age (p=0,009), occupancy density (p=0,023) with the incidence of measles outbreaks of primary school age children. Logistic regression test showed the significance value of contact history (p= 0,024), occupancy density (p=0,036), The result of in-depth interviews UKS teacher knowledge about measles is good. Conclusion of the factors that influence the incidence of measles outbreaks of primary school age children is a history of contact and occupany density with probability 83,25%. This research needs to be continued by examining other risk factors such as nutritional status, areas prone to outbreaks and implementation of the Clean and Healthy Behavior Program (PHBS) in schools.
Co-Authors Ari Rumekso Ari Suwondo Budhi R, Kamilah Cahyono - Dhetty - Olys Olys Olys, Olys Retno Wulansari Tutuk Astyawati