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All Journal Jurnal Natur Indonesia The Journal of Pure and Applied Chemistry Research
Lucy Arianie
Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

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Produksi Enzim Selulase olehAspergillus niger pada Ampas Sagu Idiawati, Nora; Harfinda, Elliska Murni; Arianie, Lucy
Jurnal Natur Indonesia Vol 16, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.239 KB) | DOI: 10.31258/jnat.16.1.1-9

Abstract

Production of cellulase by Aspergillus niger was carried out by growing the cultureson sago waste. Sago waste containscellulose that has not been used optimally. Cellulose is a polysaccharide consisting of glucose monomers linked by β-1,4-glycosides bonds. Glycoside bonds in cellulose can be enzymatically hydrolyzed into glucose with cellulase enzymes. Solid fermentation used to produce cellulase on sago waste as substrate was influenced by pH (3 to 6), moisture content(40% to 85%), and fermentation time (4 to 10 days). Products of the cellulase enzyme activity was measured by phenolsulfuricacid method. The results showed that the highest cellulase enzyme activity was 0.172 U/mL obtained at 85%moisture content, pH 5, and 8 days of fermentation time.
Dilute Ionic Liquids Pretreatment of Palm Empty Bunch and Its Impact to Produce Bioethanol Arianie, Lucy; Pebriyana, Utin Dewi; Yudiansyah, Yudiansyah; Idiawati, Nora; Wahyuningrum, Deana
The Journal of Pure and Applied Chemistry Research Vol. 3 No. 2 (2014)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1355.056 KB) | DOI: 10.21776/ub.jpacr.%y.03.02.165

Abstract

Ethanol production through ionic liquids pretreatment of palm empty bunch (PEB) was carried out. This research aims to investigate impact of ionic liquids synthetic i.e 1-butyl-3-methyl imidazoliumbromide or [BMIM]bromide toward cellulose’s palm empty bunch and convert its cellulose into bioethanol. Ionic liquid was synthesized  through reflux and microwave assisted synthesis methods. Research investigation showed that microwave assisted synthesis produce [BMIM]bromide 90% faster than reflux method. The characterization of synthesized product using FTIR, 1H-NMR, 13C-NMR and LC-MS showed that these reactions have been carried out successfully. Scanning electron microscope figure out changes morphological surface of palm empty bunch caused by ionic liquid pretreatment. Crystallinity index of PEB milled and cellulose of PEFB after [BMIM]bromide dissolution were identified using comparison of PEB FTIR spectrum. Cellulose without dilute [BMIM]bromide have higher LOI number than cellulose after [BMIM]bromide dissolution. It indicated that a large part of cellulose after dissolution has been changed into amorf. Hydrolysis residue of palm empty bunch hydrolyzed by sulfuric acids 5%, 100 0C for 5 hours and produce 685 ppm of reducing sugar. Simultaneous Saccharification and Fermentation using Trichoderma viride and Saccharomyce cerevisiae  for 5 days produce 0,69% of bioethanol.
Co-Authors Deana Wahyuningrum Elliska Murni Harfinda Nora Idiawati Utin Dewi Pebriyana Yudiansyah Yudiansyah