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All Journal Asean Journal of Science and Engineering (AJSE) ASEAN Journal for Science and Engineering in Materials
Ebulue, Maximus M.
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering

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Molecular Evaluation of Soil Respiration in Crude Oil Pollution Ebulue, Maximus M.
ASEAN Journal of Science and Engineering Vol 3, No 2 (2023): AJSE: September 2023
Publisher : Universitas Pendidikan Indonesia (UPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ajse.v3i2.45055

Abstract

Oxygen diffusion into the soil ecosystem is imperative for the sustainability of life in the soil. This study evaluated selected microbial exudates as indices of soil respiration in a crude oil polluted soil ecosystem ex-situ using biochemical and physicochemical tools to determine the following parameters; total petroleum hydrocarbon (TPH), dehydrogenases, catalase, ATP, and pH. The result of the study at 1.5 – 3.5% contamination across days-zero to -28 following crude oil impact presented an augmented increase in TPH from 0.02 ± 0.00 to 0.10 ± 0.00 with an increase in the activity of soil dehydrogenases from 4.90 ± 0.01 to 8.80 ± 0.04 katal which was significant (p0.05). Conversely, the activity of soil catalase suffered inhibition from 0.20 ± 0.00 to 0.11 ± 0.00 katal. ATP synthesis decreased from control to 0.24 ± 0.00mg/100g on week one and increased to 0.60 ± 0.10mg/100mg significantly (p0.05) on week four with contamination as the pH was reduced from 5.8 ± 0.00 to 4.1 ± 0.00 significantly, thus creating hypoxia and a reducing environment, a demonstration of soil respiration.
Physicochemical Properties of Soil Ecosystem Polluted With Spent Engine Oil Ebulue, Maximus M.; Ebulue, C. S.
ASEAN Journal for Science and Engineering in Materials Vol 1, No 2 (2022): AJSEM: Volume 1, Issue 2, September 2022
Publisher : Bumi Publikasi Nusantara

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Abstract

Assessment of the possible impact of spent engine oil on the soil ecosystem is imperative for the determination of environmental acceptability. This study investigated this impact ex-situ using standard laboratory tools to determine the following parameters: pH, temperature, moisture content, and total petroleum hydrocarbon (TPH).   The experiment demonstrated that at 1.0 – 3.5% contamination over time across days-zero to -28, spent engine oil upset the physicochemical equilibrium of the soil. A significantly reduced (p<0.05) pH in the range of 7.1 ± 0.00 to 6.2 ± 0.00 meant that the soil was acidic with a significant (p<0.05) increase in temperature from 33 ± 0.00 to 35.5 ± 0.03oC at increased concentrations overtime. The moisture content increased significantly (p<0.05) from 3.5 ± 0.00 to 19.31 ± 0.01% following the significant increase (p<0.05) in total petroleum hydrocarbon (TPH) from 0.002 ± 0.00 to 0.084 ± 0.00. This presupposed a hydrocarbon polluted soil ecosystem with oxygen deprivation; a demonstration of disturbed ecological equilibrium.
Co-Authors Ebulue, C. S.