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Lestari Indrayati, Lyna
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Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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PENETAPAN KADAR FENOLIK DAN FLAVONOID TOTAL EKSTRAK ETANOL BUAH PARIJOTO (Medinilla speciosa Blume) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Nita Damayanti, Prashinta; Putri Luhurningtyas, Fania; Lestari Indrayati, Lyna
Jurnal Farmasi (Journal of Pharmacy) Vol. 12 No. 1 (2023): Jurnal Farmasi (Journal of Pharmacy), April 2023
Publisher : Sekolah Tinggi Ilmu Kesehatan Nasional & Pengurus Cabang Ikatan Apoteker Indonesia (IAI) Sukoharjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.373013/fpnqgp60

Abstract

Parijoto (Medinilla speciosa) has a wide range of pharmacological activities due to its secondary metabolite content, especially flavonoid and phenolic compounds. Flavonoid and phenolic compounds have a major role in human health with their activities as antioxidants, anti-inflammatories, antimicrobials, and etc. The aim of this study was to determine the total levels of phenolic and flavonoid compounds from the ethanol extract of Parijoto fruit. Parijoto fruit is obtained from Bandungan, Semarang Regency, Central Java. Extraction was carried out by maceration method with 70% ethanol solvent then concentrated using a rotary evaporator. The amount of total phenolic and total flavonoid content was determined using colorimetric method with Folin-ciocalteau complex reagent on phenolic with gallic acid as reference standard and AlCl3 complex reagent on flavonoids with quercetin as reference standard. Absorbance was measured using a UV-Vis spectrophotometer. The results showed that the color of viscous extract of Parijoto fruit was dark brown with a yield of 6.67% w/w. The total phenolic content of the ethanol extract of Parijoto fruit was 21.67 µgGAE/g extract and the total flavonoid content was 9.21 µg QE/g extract.
Co-Authors Nita Damayanti, Prashinta Putri Luhurningtyas, Fania