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, Asnawatr
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Studi RegeDerasi daD Produksi Protoplas Mesofil Daun Beberapa KlOD TanamaD Kentang (Solanum tuberosum L.) . , Asnawatr; G. A. Wattimenal; M. Machmud; Agus Purwito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 3 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.148 KB) | DOI: 10.24831/jai.v30i3.1419

Abstract

The objectives of these experiments were to obtain medium composition to enlarge leaf size for protoplast production and to obtain medium composition for plant regeneration. The result showed that the best medium to produce the larger leaves was medium MS with double concentration of macronutrients without hormone supplemented with Morel vitamins, 3% (w/v) sucrose and. 7 g/l agar. This medium produced leaves with diameter of 1.44 cm comparing to control medium MS with 0.67 cm in diameter. Medium MS containing 0.1 mg/l 1AA, 0.5 mg/l Zeatin and 0.5 mg/l GA3 was able to regenerate vigorous shoots of 7 clones. Protoplast isolation of 5 clones using enzyme composition containing 0.5 % cellulase Onozuka RS, 0.05 %, pectolyase Y-23, 0.05 % MES, 9.1 % mannitol and pH 5.5, without CP W medium produced protoplast with variable yield from 10.50 x 1oJ protoplast/g leaves for Atlantic to 46.58x 1oJ protoplast/gforBF15.   Keyword: Leaves size, Plant regeneration, Potato, Protoplast i
Co-Authors Agus Purwito G. A. Wattimenal M. Machmud