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All Journal Jurnal Ilmu Dasar INDONESIAN JOURNAL OF URBAN AND ENVIRONMENTAL TECHNOLOGY
ASTRI NUGROHO
Department of Environmental Engineering, Faculty of Landscape Architecture and Environmental Technology, Trisakti University, Jakarta 11440, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Energy Environmental Science Mathematics

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Growth of Bacteria Isolat Consortium From Benakat on High Salainity Crude Oil Media Astri Nugroho; Edison Effendi; Fiona Annisa
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.222 KB)

Abstract

The production and cunsumption of oil and petroleum product are increasing worldwide and threat of oil pollution is increasing accordingly. About 10 million tons of oil and petroleum products are used worldwide each day, it will occur 5% of all oil pollution entering the sea. For that reason, needs a treatment to handle and remediate aquatic ecosystem like former. Bioremediation is a process to detocsify dan degrade crude oil. Expected from this process, land and aquatic environment where were polluted by petroleum can be natural. Treatments are divided into three stages, which are (1) isolation of dominant microorganism that degraded petroleum hydrocarbon in 15% salinity and TPH 10%, (2) Identification of bacteria with biochemistry tes and Analytical Profile Index 20 NE test (3)Biodegradation ability test in 15%, 10% salinity and 30‰ (seawater salinity) and TPH (Total Petroleum Hidrocarbon) 1%, 2.5 %, 5 %, and 10 %. The microorganisms that were identified from the isolation crude oil from Benakat are Pseudomonas aeruginosa, Aeromonas hydrophila and Agrobacterium radiobacter. Total sel microorganisms/ml in exponential phase are P. aeruginosa: 2.42E/ml, A. hydrophila: 2.99E+14 /ml, A. radiobacter: 2.42E+14/ml, and mixed culture microorganism: 4.65E/ml. The average percentage sequence degradation from the optimum  growth are mixed culture = 92%, P. Aeruginosa = 90%, A. hydrophila = 88%, and A. radiobacter = 79%. The percentage sequence degradation of mixed culture in 15% salinity and 1%, 2,5%, 5%, 10% TPH concentration are 90%, 84%, 97%, 94%. Otherwise, 30‰ salinity and 1%, 5%, 10% TPH concentration, the percentage of biodegradation are 98%, 96%, 84%.
PENGOLAHAN LIMBAH PADAT TAPIOKA MENJADI ETANOL DENGAN MENGGUNAKAN Aspergillus niger, Bacillus licheniformis DAN Saccharomyces cerevisiae Astri Nugroho; Edison Effendi; Tika Novaria
INDONESIAN JOURNAL OF URBAN AND ENVIRONMENTAL TECHNOLOGY Vol. 7 No. 1 (2015)
Publisher : Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.529 KB) | DOI: 10.25105/urbanenvirotech.v7i1.712

Abstract

Limbah padat tapioka merupakan hasil samping dari pengolahan tepung tapioka berupa ampas dan banyak mengandung karbohidrat yang dapat dikembangkan manfaatnya dengan cara mengolah limbah tersebut melalui proses hidrolisis asam dan fermentasi. Penelitian ini bertujuan mengetahui nilai C/N sebagai cara untuk mengetahui laju dekomposisi bahan organik dan mengetahui kadar etanol yang dihasilkan dari proses hidrolisis dan fermentasi pada limbah padat tapioka. Hidrolisis dilakukan dengan menggunakan 1.5% 1M H2SO4. Fermentasi dilakukan dengan variasi mikroorganisme 10% dan 20% secara mixed culture menggunakan Bacillus licheniformis, Aspergillus niger dan Saccharomyces cerevisiae pada limbah padat tapioka 50 gr, 100 gr, 150 gr dan 200 gr. Kadar etanol tertinggi pada variasi mikroorganisme 10% terjadi pada substrat 50 gr jam ke-120 yaitu 4.40% dengan jumlah mikroorganisme 2.29x1017 koloni/ml, pH = 4.60, nilai C/N = 36.43 Pada variasi mikroorganisme 20%, kadar etanol tertinggi terjadi pada substrat 50 gr jam ke-120 yaitu 3.26%, jumlah mikroorganisme 1.82x1017 koloni/ml,             pH = 3.70,  nilai C/N= 49.33. Nilai kinetika pada limbah padat tapioka 50 gr, µ = 0.003206 – 0.009712 1/jam,       µm = 0.00006120 /jam, Ks = 1036.78 mg/1, Y = 23.79, q = 0.000135 – 0.000408 1/jam, YT = 23.79 /jam,               Yobs = 1.8147 – 5.4977 /jam dan Kd = 0.0323 – 0.0388 /jam. Nilai kinetika pada mixed culture 20% pada limbah padat tapioka 50 gr, µ = 0.001818 – 0.008016 1/jam, µm=0.00004288/jam, Ks= 965.94 mg/1, Y = 7.27, q = 0.000250 – 0.001103 1/jam,  YT = 7.27 /jam, Yobs = 0.0961 – 0.4235  /jam dan Kd = 0.1296 – 0.1358 /jam.Kata kunci: Limbah padat tapioka, Bioethanol, Fermentasi, Aspergillus niger, Bacillus licheniformis, Saccharomyces cerevisiae 
Co-Authors Edison Effendi Fiona Annisa Tika Novaria