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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis
Risa Nofiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

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Characteristics of Antimicrobial Activity of Eucheuma cottonii Doty-Associated Bacteria Extracts Risa Nofiani; . Kadarisno; . Daryati; Ajuk Sapar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 12 No 2 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.983 KB) | DOI: 10.17844/jphpi.v12i2.876

Abstract

Marine organisms-associated bacteria have a potential source of antimicrobial compounds. The aim of the research was to find out characteristics of antimicrobial activity of E. cottonii Doty-associated bacteria extracts and their genus from Lemukutan Island, Kalimantan Barat. Five of 11 isolates showed antimicrobial activity namely Flavobacterium sp. K08, Alteromonas sp. K09, Bacillus sp. K10, Klebsiella sp. K11, and Pseudomonas sp. K12. Furthermore, their secondary metabolites were extracted with methanol. The characteristic of the best bacteria extracts minimum inhibitory concentrations (MICs) for Escherichia coli, Bacillus spaericus, Staphylococcus epidermidis, and Candida tropicalis were obtained from bacteria extract of Bacillus sp. K10 (250 μg/disc), Bacillus sp. K10 (750 μg/disc), Pseudomonas sp. K12, (500 μg/disc), and Alteromonas sp. K09 (1,500 μg/disc) respectively.Keywords: antimicrobial activity, Alteromonas sp., E. cottonii Doty, Flavobacterium sp., Pseudomonas sp.
ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT Risa Nofiani; Siti Nurbetty; Ajuk Sapar
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 1 No. 2 (2009): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.79 KB) | DOI: 10.29244/jitkt.v1i2.7871

Abstract

The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity. Keywords:Antimicrobial,Sponges associated bacteria,MICs
Co-Authors . Daryati . Kadarisno Ajuk Sapar Siti Nurbetty