Najeeb Mohammed Hussein
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Molecular Study Using Real Time PCR to Detect Influenza Viruses (Flu A, Flu B and RSV) in Patients at Ramadi Hospital Abbas O. Al.janabi; Mohammed Amer Fayyadh; Aseel Khalid Hameed; Najeeb Mohammed Hussein
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 2 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i2.18069

Abstract

Background: Because of the increasing incidence of infections with different types of influenza ofvarious types, type A, B, and SRV, this study included investigating the type of infection in influenzain women and men in different age groups and making a comparison between them. Methodology: 90blood samples were collected, including 30 samples for men 30 samples for women showing clinicalsymptoms of infection, and 30 samples from people with clinical symptoms under control. Thesesamples were placed in tubes containing an anticoagulant to ensure that blood does not clot DNA/RNAextraction kit, CerTest, Spanish Company and detection by Flu A, Flu B and RSV, CerTest, SpanishCompany by using real time PCR. Result: Table 1 shows the investigation of some types of influenzain women who showed symptoms of infection and who are between 20 to 40 years old, where thefrequency of infection in women between the ages of 20-30 years was high with types Flu A, SRVcompared with control and did not show infection with type B for the same Age group . As for womenaged between 30-40 years, the frequency of type B infection was high compared to control, and therewere no infections with Flu a and SRV for the same age group .
Effect of Sonchus oleracea Extract on Some Virulence Factor of Klebsiella pneumonia Which Isolated from Urinary Tract Infection in Ramadi Hospital Najeeb Mohammed Hussein; Rasool Ahmed Khalil; Ghashq Abdul Qadir Haqi Khali; Donia Kreem Mohammed Hamed
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15694

Abstract

Background: The plant contains vitamin C, thiamin, riboflavin, and some citrus and saponins. Medicalbenefits of wrapping Its medicinal benefits are attributed to the similarity between it and chicory Tonic andregulator of sugar level in The blood is a diuretic, a strong purgative, lowers the level of cholesterol in theblood and combats scurvy, as it is anthelmintic Methodology: Klebsiella bacteria were isolated from patientssuffering from urinary tract infections in Ramadi General Hospital in selective media (Maconkey Agar),four concentrations of the aqueous and alcoholic extract were made 10%, 20%, 30 % and 40% to test theeffectiveness of the extracts in inhibiting growth and virulence factors for bacteria. Result: the effect of theaqueous and alcoholic extracts of the Sonchus oleracea extract on the production of the biofilm of Klebsiellabacteria, where it was observed that there were significant differences in the concentrations of the aqueousand alcoholic extract in reducing the production of the biofilm of the bacteria, and the best concentration ofthe aqueous and alcoholic extract was 40%, where the biofilm concentration was 31 compared to the restof the concentrations 3%, 20% and 10%, where its concentration reached .55, .64, and .74, respectively,and compared to the control, where its concentration was .98%. It was not noted that there were significantdifferences between the concentrations of plant extracts and aqueous and alcoholic extract in Reducingbiofilm yield. conclusion the results explain that this plant has many It is one of the effective compoundssuch as vitamin C, thiamin, riboflavin, and some citrus and saponins. That destroy the cell wall and thusreduce the production of the biofilm and that these compounds increase by increasing the concentration ofplant extracts, and this explains the effect of the high concentration in reducing the production of the biofilm.