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All Journal International Journal of Evaluation and Research in Education (IJERE) Tropical Genetics
Abu Bakar, Adibah
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Agriculture, Biological Sciences & Forestry Education

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Development of Species-specific Cichla species eDNA Primers for Rapid Alien Invasive Species (AIS) Monitoring Osman, Nurul Fizatul Nabilah; Abu Bakar, Adibah; Saidin, Syazwan; Abdul Rahman, Ramizah; Abd Ghani, Intan Faraha; A, Amirrudin; M. N., Siti Azizah
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.21

Abstract

Peacock bass or the cichlids are known locally as top predator fishes which are invasive in Malaysia freshwater system. Detection probabilities for these fishes are typically low, especially using the conventional capture-survey method due to the fish’s behaviour of hiding beneath the water’s surface. Hence, environmental DNA (eDNA) monitoring is a relatively new non-invasive and rapid approach that can be used to assess the distribution of these invasive fishes. Here, we report the strategy i) to develop small fragment (280- 400bp) specific-specific primers for three selected invasive Cichla species namely, C. kelberi, C. ocellaris, and C. monoculus based on mitochondrial DNA (mtDNA) COI gene sequences, ii) how high quality of DNA and e-DNA should be extracted and iii) how to validate primers for specificity, (iv) developed and tested a new quantitative PCR (qPCR) assay to detect the presence of C. kelberi, and C. ocellaris, environmental DNA (eDNA) in water samples. Current research showed that the cytochrome oxidase I (COI) gene can be used to developed species- specific primers for selected species. However, several primer-designed parameters need to be adjusted to improve the specificity of primers. These were discussed in detail with future recommendations for the researcher’s references. Moreover, we found the isolation of e-DNA needs to be done within 22 days of capture to prevent false-negative results. This data can be easily applied in any fish genomic-based lab with the low-cost setting as only AGE (Agarose Gel Electrophoresis) analysis was needed for validation procedures. Overall, e-DNA analysis with species- specific primers represents new monitoring tool in detection of Aquatic Invasive Species (AIS) for management and conservation purpose.
GeneXPress card: development and evaluation of educational card game for DNA transcription subtopics in genetics Amani Norizan, Nurfarizah; Abu Bakar, Adibah; Saidin, Syazwan
International Journal of Evaluation and Research in Education (IJERE) Vol 14, No 1: February 2025
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijere.v14i1.30741

Abstract

Understanding deoxyribonucleic acid (DNA) transcription poses a substantial challenge in studying genetics, often due to lack of understanding and traditional teaching methods that fail to engage students. This study introduces GeneXPress, an innovative card game, aimed at enhancing the learning experience of DNA transcription for higher education biology students. Using the ADDIE model, this preliminary study assessed the usability of the GeneXPress card game through a survey of 169 biology education students at Universiti Pendidikan Sultan Idris, Malaysia. A quantitative approach was employed to analyze the survey data, evaluating the game’s usability using descriptive statistics, including mean scores and standard deviations. The findings reveal high usability and positive views of GeneXPress, covering various aspects such as goals, design, components and organization, playability and usefulness, with average scores of above 3.79. The positive perception of GeneXPress among students highlights its ability to engage and motivate learners, making the study of complex genetic concepts more accessible and enjoyable. In conclusion, it underscores the vital role of innovative teaching aids in enhancing student engagement and understanding, paving the way for future explorations into educational tools that cater to the evolving needs of learners and educators alike.
Co-Authors A, Amirrudin Abd Ghani, Intan Faraha Abdul Rahman, Ramizah Amani Norizan, Nurfarizah M. N., Siti Azizah Osman, Nurul Fizatul Nabilah Saidin, Syazwan