Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
IDA HANARIDA SOMANTRI
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Economics, Econometrics & Finance

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Regeneration Capacity of Indica Rice cv. Bengawan Solo in Two Types of Regeneration Media through Microprojectile Bombardment TRI JOKO SANTOSO; SUDARSONO SUDARSONO; HAJRIAL ASWIDINNOOR; IDA HANARIDA SOMANTRI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.957 KB) | DOI: 10.4308/hjb.12.4.157

Abstract

Factors affecting the success of gene delivery into cells/tissues of rice mediated by particle bombardment have been investigated. However, their effects on regeneration capacity of rice explants have not been investigated. Objectives of this experiments were to evaluate the effects of particle bombardment on regeneration capacity of indica rice cv. Bengawan Solo calli and the effects of four regeneration media on the development of bombardment calli. Calli of indica rice were initiated from mature seeds on callus-inducing medium. They were transferred on osmoticum containing medium prior to bombardment and maintained on the medium up to two days. Particle bombardment was conducted at 16 cm bombardment distance, 27 cm Hg helium pressure and using standard procedures for preparation of microprojectile particles. The bombarded calli were cultured on RNB or RMS regeneration medium, with or without spermidine addition. Results of the experiment indicated particle bombardment affected the survival of explants of indica rice but did not affect the development of the explants. The development of explants was rather dependent on the regeneration media than on the particle bombardment treatment. RMS medium was better than RNB medium for shoots inducing from bombarded indica rice explants.
Kultur Antera, Teknik Penyelamatan Embrio dan Rekayasa Genetik untuk Menunjang Pemuliaan Tanaman Padi Ida Hanarida Somantri; A. Dinar Ambarwati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1205.575 KB) | DOI: 10.24831/jai.v29i3.1544

Abstract

The success of biotechnology in developed country encourage Indonesia to use it in rice breeding program. Every technick In biotechnology has advantage and it can be used to solve a problem in a certain purpose. Embryo rescue technich solved the problem of crossing with wild species. This technick may insert a gene from one organism to another through gene  transformation technich. The anther culture technich been used on rice: Javonica, Javanica and indica. Generally, the percentage of callus induction and regeneration has great variation (1.8% - 40%). It was Influenced by the number and type of genome. The transformation of rice using microprojecty system using cry gene resulted 172 putative transgenic plants. Twenty five of them have been tested using moleculer technick and it showed t at the plants contained cry gea.   Key words: Anther culture, Embryo rescue, Rice
Co-Authors A. Dinar Ambarwati HAJRIAL ASWIDINNOOR Sudarsono Tri Joko Santoso