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SUSUMU SHIRAISHI
Graduate School of Bioresource and Bioenvironmental Science, Kyushu University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Genotype identification of Sengon (Paraseriathes falcataria (L.) Nielsen needs accurate and reliable method for identification. Sixteen individuals of P. falcataria from Okinawa, Japan were used as materials for selecting polymorphic RAPD fragments and for developing SCAR marker whereas confirmation of polymorphisms of the SCARs was conducted using 24 materials from Candiroto Seed Orchard, Indonesia. Forty-eight polymorphic fragments were obtained from screening in 288 RAPD primers. Forty-six ou VIVI YUSKIANTI; SUSUMU SHIRAISHI
HAYATI Journal of Biosciences Vol. 17 No. 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.4.167

Abstract

Genotype identification of Sengon (Paraseriathes falcataria (L.) Nielsen needs accurate and reliable method for identification. Sixteen individuals of P. falcataria from Okinawa, Japan were used as materials for selecting polymorphic RAPD fragments and for developing SCAR marker whereas confirmation of polymorphisms of the SCARs was conducted using 24 materials from Candiroto Seed Orchard, Indonesia. Forty-eight polymorphic fragments were obtained from screening in 288 RAPD primers. Forty-six out of 48 SCAR primers were successfully developed. Examination of the newly-designed of SCAR primer using 24 P. falcataria from Candiroto Seed Orchard, Indonesia found 5 polymorphic SCAR primers. These five SCARs are considered as useful marker for genotype and clone identification within P. falcataria.
Co-Authors VIVI YUSKIANTI