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DEWI SUKMA
Bogor Agricultural University
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Phalaenopsis is one of the most interesting genera of orchids due to the members are often used as parents to produce hybrids. The establishment and development of highly reliable and discriminatory methods for identifying species and cultivars has become increasingly more important to plant breeders and members of the nursery industry. The aim of this research was to develop sequence-based microsatellite (eSSR) markers for the Phalaenopsis orchid designed from the sequence of GenBank NCBI. Seve . FATIMAH; DEWI SUKMA
HAYATI Journal of Biosciences Vol. 18 No. 2 (2011): June 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.2.71

Abstract

Phalaenopsis is one of the most interesting genera of orchids due to the members are often used as parents to produce hybrids. The establishment and development of highly reliable and discriminatory methods for identifying species and cultivars has become increasingly more important to plant breeders and members of the nursery industry. The aim of this research was to develop sequence-based microsatellite (eSSR) markers for the Phalaenopsis orchid designed from the sequence of GenBank NCBI. Seventeen primers were designed and thirteen primers pairs could amplify the DNA giving the expected PCR product with polymorphism. A total of 51 alleles, with an average of 3 alleles per locus and polymorphism information content (PIC) values at 0.674, were detected at the 16 SSR loci. Therefore, these markers could be used for identification of the Phalaenopsis orchid used in this study. Genetic similarity and principle coordinate analysis identified five major groups of Phalaenopsis sp. the first group consisted of P. amabilis, P. fuscata, P. javanica, and P. zebrine. The second group consisted of P. amabilis, P. amboinensis, P. bellina, P. floresens, and P. mannii. The third group consisted of P. bellina, P. cornucervi, P. cornucervi, P. violaceae sumatra, P. modesta. The forth group consisted of P. cornucervi and P. lueddemanniana, and the fifth group was P. amboinensis.
THE EFFECT OF GAMMA IRRADIATION TO THE PHENOTYPIC OF TWO AGLONEMA VARIETIES Arya Widura Ritonga; Dewi Sukma
Agrotech Journal Vol 2, No 2 (2017): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v2i2.312

Abstract

Increased phenotypic diversity is needed to increase the economic value of Aglaonema. However, information on increasing phenotypic diversity of Aglaonema using gamma-ray irradiation has not been widely known. This study aimed to investigate the effect of gamma ray irradiation treatment to the performances of two varieties of Aglaonema. This research was arranged factorially using randomized group design (RKLT) of two factors consisting of 8 combinations of treatments that are 4 level of irradiation dose and 2 Aglaonema varieties. The results showed that the induction of gamma ray irradiation decreased the % viable of the plants, the number of leaves, leaf length, leaf width, and the % green color as well as increased the % blue on the leaves of Aglaonema Butterfly and Aglaonema Siam Aurora. The interaction between dose of irradiation and aglaonema varieties was obtained in the % red of leaf color. Both of Aglaonema varieties had a high radiosensitivity with LD50 values ranged of 16.70 - 17.14 Gy
Co-Authors . FATIMAH Ritonga, Arya Widura