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All Journal HAYATI Journal of Biosciences Majalah Kedokteran Bandung Folia Medica Indonesiana
Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Medicine & Pharmacology Other

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Potential of Conditioned Medium of hATMSCs in Aging Cells Model Wahyu Widowati; Rachmawati Noverina; Wireni Ayuningtyas; Dedy Kurniawan; Seila Arumwardana; Hanna Sari W Kusuma; Rizal Rizal; Dian Ratih Laksmitawati; Ratih Rinendyaputri; Rilianawati Rilianawati; Ahmad Faried
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.378-388

Abstract

Skin aging is caused by the exposure cumulative of ultraviolet radiation, it leads reactive oxygen species (ROS) production in the skin. The conditioned medium of human Adipose Tissue-derived Mesenchymal Stem Cells (hATMSCs) can scavenge free radicals and increase the survival rate of skin cells under oxidative stress. This study examined the protective effects of Conditioned Medium (CM) of hATMSCs in H2O2-induced human skin fibroblast cell line (BJ). The aging cells model using H2O2-induced BJ cells were added CM-hATMSCs in concentrations (0, 10, 30%) and incubated in various time, furthermore BJ cells induced by various H2O2 concentrations (0, 50, 100, 200 µM) incubated for 1 h. The anti-aging potential were measured including viability, ROS and collagen levels in BJ cells which treated CM-hATMSCs. The median inhibitory concentration (IC50) of H2O2 on BJ cells for 1 h incubation was 107.87 μM and 91.25 μM for 10 min incubation. CM-hATMSCs increased the viability on aging model cells. CM-hATMSCs concentration 30% increased the viability of H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs concentration 25% decreased ROS, increased collagen level in H2O2 50, 100, 200 µM-induced BJ cells. CM-hATMSCs increase the viability cells, collagen level and decrease ROS level in aging model cells.
Antioxidant Activities of Ginger (Zingiber officinale) and Telang Flower (Clitoria ternatea L.) Combination Tea Wahyu Widowati; Teresa Liliana Wargasetia; Teddy Marcus Zakaria; Meganita Marthania; Michael Sebastian Gunadi; Nathanael Halim; Nindia Salsabila Mia Dewi; Sherly Santiadi
Majalah Kedokteran Bandung Vol 54, No 3 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n3.2729

Abstract

Tea is widely consumed for health purposes to prevent and treat various diseases. This paper reports the antioxidant activities of a herbal tea namely jahe (ginger, Zingiber officinale) telang (Clitoria ternatea L.), and the combination namely JaTe (jahe and telang). Each of jahe, telang, and JaTe teas were made of 2g ginger, 5 buds dried telang flower, and the combination respectively. The teas were boiled in 200 mL for 5 minutes. The dried ginger, telang, and JaTe formulation were evaluated for antioxidant activities. Total phenolic content was evaluated by Folin-Ciocalteu using the gallic acid equivalent (GAE), while total flavonoid content was evaluated by aluminum chloride colorimetric assay using the quercetin equivalent (QE). The antioxidant activity was measured using 2,2-diphenyl-1picrylhydrazyl (DPPH), and 2,2'-Azino-Bis-3-Ethylbenzothiazoline-6-sulfonic acid (ABTS), hydrogen peroxide (H₂O₂) scavenging activities, and ferric reducing antioxidant power (FRAP) assay. The highest phenolic and flavonoid contents were found in ginger tea with 23.62 μg GAE/100% concentration and 7.22 μg QE/100% concentration, followed by telang tea with 16.20 μg GAE/100% concentration and 4.88 μg QE/100% concentration. Ginger tea has the strongest DPPH scavenging activity with IC₅₀=7.76% followed by telang tea 17.07%. JaTe tea has the strongest ABTS scavenging activity with IC₅₀=1.01% followed by ginger tea with IC₅₀=1.26%. Ginger tea has the strongest H₂O₂ scavenging activity with IC₅₀=13.23% and followed by JaTe tea with IC₅₀=14.66%. JaTe tea has the strongest FRAP activity with IC₅₀=3.59%, followed by ginger tea with IC₅₀=4.94%. Overall, JaTe herbal tea formulation has a strong antioxidant activity.
An in Vitro Assay Reveals the Anti-Aging Properties of Temulawak Extract (Curcuma xanthorrhiza L.) Teresa Liliana Wargasetia; Wahyu Widowati; Anis Syabani Muthmainnah; Rizal
Folia Medica Indonesiana Vol. 59 No. 4 (2023): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i4.44944

Abstract

Highlights:1. This study investigated the potential of temulawak extract as a cost-effective option for cosmeceutical applications in the treatment of aging. 2. Temulawak extract was found to have the ability to inhibit elastase, hyaluronidase, and tyrosinase activities, thus making it a suitable option for cosmeceutical applications.   AbstractAging is the gradual loss of a tissue's capacity to heal and maintain normal or physiological form and function. Elastase, hyaluronidase, and tyrosinase are the enzymes that contribute to the process of skin aging. The anti-aging effect is connected to the inhibition of these enzymes' activities. Numerous medicinal plants with active metabolites have been extensively utilized to treat aging. The active compounds of temulawak (Curcuma xanthorrhiza L.), such as phenolics, curcuminoids, and xanthorrhizol, have promising properties that may be used as anti-aging agents. This study aimed to determine whether temulawak extract, a potential option for cosmeceuticals, has anti-aging properties that can inhibit the enzymes elastase, hyaluronidase, and tyrosinase. A stock solution was made by dissolving 20 mg of temulawak extract in 1 mL of 100% dimethyl sulfoxide (DMSO). The stock solution was then diluted to produce working solutions with concentrations ranging from 31.25 to 1000 μg/mL. An in vitro assay was carried out in three replications to examine the anti-aging activity of the temulawak extract. The in vitro assay investigated the inhibition of the enzyme elastase, hyaluronidase, and tyrosinase at seven different concentrations, with the following ranges: 2.08–66.67 μg/mL for the anti-elastase, 5.21–166.67 μg/mL for the anti-hyaluronidase, and 3.125–100 μg/mL for the anti-tyrosinase. IBM SPSS Statistics for Windows, version 20.0 (IBM Corp., Armonk, N.Y., USA) was used to perform the statistical analysis, with a significance level of p<0.05. Temulawak extract exhibited the highest inhibition rates, reaching 82.72%, 89.41%, and 94.17% for the anti-tyrosinase, anti-elastase, and anti-hyaluronidase activities, respectively. The median inhibitory concentrations (IC50) were 10.66, 70.39, and 55.87 μg/mL for the elastase, hyaluronidase, and tyrosinase activities, respectively. This study revealed that temulawak extract has strong anti-aging properties as it effectively inhibits the activities of elastase, tyrosinase, and hyaluronidase. In conclusion, temulawak extract can be considered a promising candidate for cosmeceutical applications.   
Co-Authors Ahmad Faried Anis Syabani Muthmainnah Dedy Kurniawan Dian Ratih Laksmitawati Hanna Sari W Kusuma Meganita Marthania Michael Sebastian Gunadi Nathanael Halim Nindia Salsabila Mia Dewi Rachmawati Noverina Ratih Rinendyaputri Rilianawati Rilianawati Rizal Rizal Rizal Seila Arumwardana Sherly Santiadi Teddy Marcus Zakaria Teresa Liliana Wargasetia Wireni Ayuningtyas