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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Tropical Animal Science Journal

P. D. M. H. Karti

Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University

Author-ID : 2425321

Agriculture, Biological Sciences & Forestry Energy

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Improvement of Indigofera zollingeriana Production and Methionine Content through Inoculation of Nitrogen-Fixing Bacteria P. S. Hutapea; L. Abdullah; P. D. M. H. Karti; I. Anas
Tropical Animal Science Journal Vol. 41 No. 1 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Indigofera zollingeriana is a tremendous potential as a forage plant due to its high protein content. This experiment was conducted to increase production and nutrient quality of I. zollingeriana biomass, especially methionine content through inoculation of nitrogen fixing bacteria. Nine isolates obtained from nodule of I. zollingeriana were screened for 37 days in greenhouse, resulting in three desirable isolates. These three selected isolates at density of 105 cfu/mL and 107 cfu/mL were inoculated as much as 1 mL per seedling. Uninoculated plants were also grown as control treatment, i.e., positive (+ 0.05% KNO3 (w/v)) and negative. The experiment was laid out in a completely randomized design with four replicates and carried out for three months. Plant biomass was analyzed for amino acid and other nutrient contents. Moreover, nodulation activity was also observed by counting and weighting the nodules. The results showed that Bradyrhizobium sp. P8 828, Bradyrhizobium sp. PZS_A08, and Roseomonas sp. CMS4Y-2-2 significantly increased shoot production. Subsequent experiment showed that plant inoculation with Bradyrhizobium sp. PZS_A08 at 105 cfu/mL significantly increased dry matter content. Inoculation with Bradyrhizobium sp. PZS_A08 and Roseomonas sp. CMS4Y-2-2 at 107 cfu/mL produced higher crude protein contents that were better than the application of nitrogen fertilizer. Inoculation treatment with Bradyrhizobium sp. P8 828 at 105 cfu/mL was the most effective in improving methionine content. It is concluded that production and quality of biomass obtained from inoculation treatments were comparable to the application of inorganic N-fertilizer.

Evaluation of Arbuscular Mycorrhizal Fungi Inoculum on Production and Nutrient Content of Pennisetum purpureum P. D. M. H. Karti; I. Prihantoro; M. A. Setiana
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Land for forage planting is mainly on marginal land such as acid soil. However, the constraint is the low levels of phosphorus (P) that can inhibit forage production. Arbuscula mycorrhizal fungi has been known as a biological fertilizer because the fungi can help the absorption of phosphorus (P) on the root so that can improve the forage production and quality of nutrients. This study was aimed to utilize and evaluate the use of arbuscular mycorrhizal fungi inoculum in forage production and nutritive value of Pennisetum purpureum. The experiment used a completely randomized design with two factors (2 x 4) and 4 replications. The first factor was type of AMF inoculum (A and B) and the second factor was doses of AMF (D1= 0.5 kg/planting hole, D2= 1 kg/planting hole, D3= 1.5 kg/planting hole, and D4= 2 kg/planting hole). Control treatment was carried out separately. The result showed that the highest shoot dry weight production was on AD2 and significantly different (P<0.05) from BD1 and control. There was no interaction between type and dose of inoculum on shoot dry weight production and nutrition value. The inoculum A significantly increased (P<0.05) shoot dry weight production (34.04%), crude protein content (10.21%), phosphorus uptake (40%), N content (10.53%), N uptake (38.10%), and protein production (40.15%) of P. purpureum, compared to inoculum B. It can be concluded that AMF inoculum type A was the best inoculum for forage production.

Co-Authors I. Anas I. G. Permana I. Prihantoro K. G. Wiryawan L. Abdullah M. A. Setiana M. Telleng P. S. Hutapea V. E. Se'u

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