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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Tropical Animal Science Journal

C. Sumantri

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University

Author-ID : 2425592

Agriculture, Biological Sciences & Forestry Energy

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Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep A. Gunawan; D. Anggrela; K. Listyarini; M. A. Abuzahra; Jakaria Jakaria; M. Yamin; I. Inounu; C. Sumantri
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.

The Prediction of Prolificacy Using Linear Body Parameters and Craniometric Analysis in Etawah-Grade Does R. H. Mulyono; C. Sumantri; R. R. Noor; Jakaria Jakaria; D. A. Astuti
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Etawah-grade goat is a graded up line between Kacang and Etawah goats, which well adapted to Indonesia’s humid tropical climate. In order to fulfill national meat requirement, it is necessary to increase the population by increasing prolificacy. This study was aimed to determine whether the body and head measurements of the Etawah-grade does can be used as selection criteria for indirect selection of the litter size. The use of does at 3-4 years old (I3 dental condition) from BPTU-HPT Pelaihari and Cordero farms (51 and 55 does, respectively) were based on their specific geographical location characteristics. The body variables were withers height, hip height, body length, chest width, chest depth, thurl width, rump length, chest girth, and cannon circumference, whereas for head variables were acrocranion–prosthion, basion–prosthion, lower jaw length, head height, tuber facial left-right, nasion–rhinion, entorbitale left-right, euryon left-right, supraorbitale left-right. Bergmann methods were applied for measuring the differences of body and head parameters followed by the principal component, Fisher’s discriminant, and principal component regression analyzes. All regression coefficients of linear body measurementss were highly correlated to the litter size (P<0.01), with the equation was Y= 0.015X1b + 0.011X2b + 0.025X3b - 0.002X4b + 0.022X5b + 0.010X6b + 0.022X7b + 0.030X8b + 0.026X9b. The chest girth was the most elastic body size measurement to the litter size. The increasing of 1 cm of doe’s chest girth would result in the increase of litter size, each of 0.0545 and 0.0417 heads born-1 at BPTU-HPT Pelaihari and Cordero farms. Differences in head size and head shape due to the morphometric adaptations cannot be used to predict litter size.

Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour K. Listyarini; Jakaria Jakaria; M. J. Uddin; C. Sumantri; A. Gunawan
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) and kinesin-like protein KIF12 (KIF12) genes are predicted as candidate genes which play important roles in lamb flavour and odour. The aim of this study was to analyse the genotype polymorphism of CYP2A6 and KIF12 genes, to study association and expression of these genes with lamb flavour and odour. Identification of genes polymorphism and associations of CYP2A6 and KIF12 genes were performed using PCR-RFLP method and GLM analysis. The PCR-RFLP products of CYP2A6 and KIF12 were digested by restriction enzyme BsmAI and BfaI, respectively. The expression of CYP2A6 gene was performed using qRT-PCR. The results showed that the CYP2A6 and KIF12 genes were polymorphics. The CYP2A6 gene found to have two genotypes (TT and GT), whereas the KIF12 gene found to have three genotypes (CC, CT, and TT). The CYP2A6 and KIF12 genes were in Hardy Weinberg Equilibrium (HWE). Association analysis showed that CYP2A6 (g.49170107 G>T) was significantly (P<0.05) associated with 3-methylindole (MI) or skatole, while KIF12 (g.9617965 C>T) was not significantly associated with lamb flavour and odour. The GT genotype exhibited a greater 3-methylindole (MI) or skatole than the TT genotype (P<0.05). The mRNA expression analysis showed that CYP2A6 mRNA expression was higher (P<0.01) in animals with the TT genotype. These results will improve the understanding of the functions of the CYP2A6 in lamb flavour and odour, especially in term of 3-methylindole (MI) or skatole compound within the liver and will shed light on CYP2A6 as a candidate in the selection of sheep with low lamb flavour and odour.

Co-Authors A. Gunawan Asep Gunawan C. Budiman D. A. Astuti D. Anggrela I. I. Arief I. Inounu I. Khaerunnisa Jakaria Jakaria K. Listyarini K. Listyarini M. A. Abuzahra M. Indriati M. J. Uddin M. Yamin R. H. Mulyono R. R. Noor T. Susanti

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