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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal Bioma : Jurnal Biologi Indonesia Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Jurnal Agripet Animal Production
Asep Gunawan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Identification of Body Size and Body Shape of Garut Sheep Fighting Type and Meat Type and Garut Cross Based on Principal Component Analysis Gunawan, A; Mulyono, RH; Sumantri, C
ANIMAL PRODUCTION Vol 11, No 1 (2009): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.712 KB)

Abstract

This research was done to identify the body size and shape  among three types of local sheeps of Garut fighting type, Garut meat type and their crossbred fighting and meat type in Garut district (Margawati, Sukawening and Wanaraja) and Bogor district (Ciomas and Cinagara).  The total number of sheep from Garut district used in this study were 520 head consisting of crossbred sheep from Margawati (71 head), fighting sheep from Wanaraja (79 head), meat sheep from Wanaraja (84 head), fighting sheep from Sukawening (87 head) and meat sheep from Sukawening (62 head). Whereas the total number of sheep from Bogor district were 137 head consisting of those of fighting sheep from Ciomas (66 head) and from Cinagara (70 head). Some body measurenments measured in this study were body weight, body length, wither height, chest width, chest circumfrence, cranium, tail length and tail width.  Analysis of all those sizes were based on principal component analysis (PCA) then visualized into the group of crowded diagram.  Different group of crowed builded on the base of scoring in body size and body shape derivated from covarian matrix were able to identifed morphologycal penotypic differences among garut sheep studied.   The results from canonical  analyses showed that body lenght, chest girth,chest width, and wither height were the most discriminant variables had impact to the diferences score size between  types of Garut sheep. However, the tail length and tail width were variables had impact to diferences score shape. The  crowed diagram showed that an intersection among three types (fighting, meat and crossbred) of sheeps suspected as resulted from the same genetic resource, namely Garut sheep.  Genetic flow was suspected from crossbred in Margawati to meat sheep in Cinagara as well as the sheeps from Sukawening and Wanaraja to fighting sheep in Ciomas.  Close genetic distances that were found among fighting and meat sheeps in Sukaweining as well as fighting and meat sheeps in Wanaraja could be considered that these two groups of sheeps as the genetic resource of fighting sheep in Ciomas. (Animal Production 11(1): 8-14 (2009) Key Words: garut sheep, principal component analysis (PCA), body size and shape
Pendugaan Nilai Heritabilitas Bobot Lahir dan Bobot Sapih Domba Garut Tipe Laga A Gunawan; R R Noor
Media Peternakan Vol. 29 No. 1 (2006): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objective of this study was to estimate heritability of birth and weaning weights of the fighting type of Garut sheep. The data of birth and weaning weight of 175 SR (Super lambs, i.e. cross between fighting type Garut rams and selected Garut ewes) and 61 SB (Sukabumi lambs, i.e. cross between fighting type Garut rams and Sukabumi ewes) were used. The data were collected from March 2001 to August 2002. The results showed that the means of all traits of SR sheep group were larger (P < 0.01) than those of SB sheep group. Estimated heritability of birth weight and weaning weight of SR sheep were 0.67  ± 0.19 and 0.95  ± 0.16 respectively. Estimated heritability value of birth and weaning weight of SB sheep were 0.53  ± 0.33 and 0.57  ± 0.37 respectively. The heritability of birth and weaning weight were considered as high which means that the selection programme will be more effective and efficient in improving the genetic merits. Key words : heritability, birth and weaning weight, fighting type Garut sheep
Genetic and Phenotypic Parameters of Body Weight in Ettawa Grade Goats F Hasan; . Jakaria; A Gunawan
Media Peternakan Vol. 37 No. 1 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.382 KB) | DOI: 10.5398/medpet.2014.37.1.8

Abstract

The aim of this study was to estimate genetic and phenotypic parameters of growth traits consisting of birth weight (BW), weaning weight (WW), 6 mo body weight (6WM), 12 mo body weight (12WM), and 18 mo body weight (18WM) of Ettawa Grade goats. The number of goat used to determine growth traits of BW, WW, 6WM, 12WM, and 18WM were 316; 316; 259; 259 and 165 heads, respectively. Data were analyzed using General Linear Model (GLM) to identify non-genetic effect. Estimation of genetic and phenotypic parameters including heritability, repeatability, genetic and phenotypic correlation were calculated using Restricted Maximum Likelihood and GLM. Genetic trends were calculated using the regression of mean breeding values on birth year. The results showed that parity and type of birth had significant (P<0.05) influence on all growth traits. Estimated heritability of birth, weaning, 6WM, 12WM, and 18WM were 0.54±0.12; 0.35±0.07; 0.37±0.09; 0.68±0.16 and 0.63±0.19, respectively. Estimated repeatability of WW, 6WM, 12WM and 18WM, WW, 6WM, 12WM and 18WM were 0.98±0.01; 0.97±0.01; 0.94±0.03; 0.71±0.12 and 0.91±0.04, respectively. The genetic trends for traits of BW and 18MW were decreased fluctuatively. However, the WW, 6MW, 12MW were increased fluctuatively. The high and positive genetic correlations between all growth traits and 12WM traits in this study indicated that selection for high 12WM will improve genetic merit in Ettawa Grade goats. Key words: body weight, EBV, Ettawa Grade goat, genetic and phenotypic parameters
Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle R. Putri; R. Priyanto; A. Gunawan; . Jakaria
Media Peternakan Vol. 38 No. 3 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (747.597 KB) | DOI: 10.5398/medpet.2015.38.3.145

Abstract

Calpastatin (CAST) gene is well known as an inhibitor of muscle protein degradation and relates to muscle growth and meat tenderness. The objective of this study was to determine the association of CAST gene with growth traits and carcass characteristics in Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali Cattle to obtain growth traits, carcass characteristics, and blood samples. Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA sequencing. The association of CAST gene with growth traits and carcass characteristics were analyzed by using General Linear Model (GLM). The result showed that there were two genotypes (GG and AG) of CAST gene with allele frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG of CAST gene significantly affected (P<0.05) the back-fat thickness and longissimus dorsi without a significant effect on the growth traits. It could be concluded that CAST gene had a potency as a marker gene for carcass quality in Bali cattle.  Key words: Bali cattle, CAST gene, PCR-RFLP, polymorphism
Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle Himmatul Khasanah; A. Gunawan; R. Priyanto; M. F. Ulum; Jakaria Jakaria
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.226 KB) | DOI: 10.5398/medpet.2016.39.2.95

Abstract

Myostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P≤0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P≤0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality.
Sifat-Sifat Kuantitatif dan Jarak Genetik Kerbau Lokal Sulawesi Tenggara Berdasarkan Pendekatan Morfologi Muh. Rusdin; Dedi Duryadi Solihin; Asep Gunawan; Chalid Talib; Cece Sumantri
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 3 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.318 KB) | DOI: 10.18343/jipi.23.3.203

Abstract

Information on animal genetic resource is needed in conservation program of Indonesian local buffalo. This study was aimed to analyze the quantitative traits diversity and genetic distance of local buffalo of Southeast Sulawesi local based on morphological approach. The total of 271 heads of swamp buffaloes were used in the study from four sub-populations, namely Bombana island’s, Bombana mainland, Kolaka and Konawe. Duncan Multiple Range test at 5% probability was used to compare the quantitative traits between the buffalo sub-populations. Genetic distance was analyzed by using the simple discriminant function. The results showed that the average body weight of male buffalo between sub-populations was not significantly different (P>0.05), but the average body weight of females buffalo from Bombana Island’s (465.22 ± 103.25) was significantly (P<0.05) higher than those of the other sub-populations. The highest variation of quantitative traits of local buffalo was found in Konawe sub-population, i.e. in body weight of female buffalo (35.36%) and body weight of male buffalo (22.05%), rump length in males buffalo (20.44%), and rump width in females buffalo (14.58%). Southeast Sulawesi local buffalo can be grouped into three clusters based on the phylogeny tree, namely 1) Kolaka and Konawe cluster, 2) Bombana island’s cluster, and 3) Bombana mainland cluster. The distinguishing variables of the group of Southeast Sulawesi local buffalo were rump length, body length, and chest circumference. The highest phenotype similarity value was shown by Bombana island’s buffalo (68.57%), while the lowest was found at Konawe buffalo (26.76%). The farthest genetic distance was found between Bombana Islands and Konawe buffalo (3.71703), while the closest genetics distance was identified between Kolaka and Konawe buffalo (0.86616).
Karakteristik Morfometrik Ukuran Tubuh dan Bentuk Domba Ekor Gemuk Pulau Madura dan Rote dengan Menggunakan Analisis Komponen Utama A. Gunawan; C. Sumantri
Buletin Peternakan Vol 31, No 4 (2007): Buletin Peternakan Vol. 31 (4) November 2007
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v31i4.1236

Abstract

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Thermoregulation, Haematological Profile and Productivity of Holstein Friesian Under Heat Stress at Different Land Elevations Elmy mariana; Cece Sumantri; Dewi Apri Astuti; Anneke Anggraeni; Asep Gunawan
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.37648

Abstract

The purpose of this research was to determine the effect of heat stress on thermoregulation, haematology, and productivity of Holstein Friesian (HF) dairy cows raised in different elevations. A total of 63 HF in a normal lactation period were used in this study. The research was conducted for 3 months during dry season in three different areas, which were at Pondok Ranggon (97 m.a.s.l) which categorized as a lowland, Ciawi (576 m.a.s.l) which categorized as a lower-upland, and Lembang (1241 m.a.s.l) which categorized as an upland. Observation on microclimate aspects which includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) was done by recording each variable for every 2 hours starting from 08.00 to 16.00 WIB. The thermoregulation analysis was done based on the given physiological responses which consisted of the skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr), Heat Tolerance Coefficient (HTC), plasma cortisol level and haematological profile observation. The Ta, Rr, and THI measurements showed that in the lowland and lower-upland, the HF experienced moderate heat stress, while the HF raised in the upland area experienced less heat stress. The results showed that the dairy cows which raised in lowland had the highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). All of the physiological and haematological parameters in the three study area showed a normal value.Furthermore, the erythrocyte, Hb and PVC concentration in a lowland raised HF were higher (P<0.05), while the plasma cortisol levels were not significantly different. The milk production of the observed dairy cows in different elevations was significantly different (P<0.05), with the highest milk yields, were found in the upland raised HF (13.1±3.52 kg), followed by the lower-upland (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, all of the HF raised in different land elevations was exposed to heat stress during dry seasons, even though the cows showed the ability to physiologically adapt and cope with the conditions. 
Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep A. Gunawan; D. Anggrela; K. Listyarini; M. A. Abuzahra; Jakaria Jakaria; M. Yamin; I. Inounu; C. Sumantri
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.335 KB) | DOI: 10.5398/tasj.2018.41.3.165

Abstract

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.
Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens M. Ardiyana; A. Gunawan; S. Murtini; T. Sartika; C. Sumantri
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.018 KB) | DOI: 10.5398/tasj.2020.43.2.95

Abstract

NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.
Co-Authors A. Furqon A. S. Mentari Ahmad Furqon Akbanugra Yudhananda Amri, Fisma Anneke Anggraeni Astira, Adinda Wanda C Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Talib Cahyo Budiman Cece Sumanri Cece Sumantri Chalid Talib D. Anggrela D. D. Solihin Dedi Duryadi Solihin Dedi Duryadi Solihin Depison Depison Depison Depison DEWI APRI ASTUTI Elmy mariana F Hasan Gushairiyanto Gushairiyanto Hanum Isfaeni Herpina Herpina Himmatul Khasanah I. Inounu Ismeth Inounu J. P. Munyaneza Jakaria Jakaria Junaedi Junaedi K. Listyarini K. Listyarini Katrin Roosita Kusnadidi Subekti Listyarini, Kasita M. A. Abuzahra M. Ardiyana M. F. Al-Habib M. F. Ulum M. J. Uddin M. Yamin Ma'arif, Naufal Mohamad Yamin Mohamad Yamin Muh. Rusdin Muhammad Suhendra, Muhammad N. Ulupi Naufal Ma'arif Niken Ulupi Novita Nur Anggraeni R R Noor R. Iis Arifiantini R. Priyanto R. Putri R. R. Noor R. R. Noor Ratna Sholatia Harahap Raudah Putri Ekawati RH Mulyono, RH Ronnie Permana Ronny Rachman Noor Rudi Afnan Rudy Priyanto S. Murtini S. Murtini S. Y. Basril Septiyawan, Ganda Adi Shidiq, Fajrin SRI RAHAYU T. Sartika Tuti Suryati Widya Pintaka Bayu Putra Woki Bilyaro Yulianto Yulianto Yun Alwi Yusuf, Salman