Rawaa M. Mohammed
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The Effects of Mouth Bacterial Infection on Saliva Flow Mohammed F. Jubaz; Rawaa M. Mohammed; Syoof Khoman Alwan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14497

Abstract

Background: Mouth bacterial infections most probably due to dental cares are a prevalent disease. Salivaryflow rate is affected by many diseases but there is not enough published data about the effect of mouthbacterial infection on the salivary flow rate. Objective: this study examines the relationship between salivaryflow rate and mouth bacterial infection in adult population. Methodology: cross sectional study. Result:salivary flow rate is affected by mouth bacterial infection as it is founded to be very low flow rate in 17% andlow flow rate in 40% of the sample population. Conclusion Salivary flow rate is affected by oral bacterialinfections that need urgent medical intervention to improve the outcome of those patients.
Isolation of Bacteria and Detection of Cephalosporin Antibiotics Effects among Patients with UTI in al-hilla City Rawaa M. Mohammed; Asma’a HM
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14505

Abstract

90 urine samples were collected randomly from patients whose visit the Urology department in MurjanMedical City in Al-Hilla in period between April to July 2020 , the patients included 35 males and 55females and in age between 17-77 years old. The urine samples divided into two parts , the first part using forgeneral urine examination and the second part using for culture and sensitivity . The results of current studyappeared to 35 (39%) males among total patients infected with UTI while 55(61%) females among totalpatients infected with UTI . In field of effects of age on infection the current results appeared in females theinfection of urinary tract increase in young age group (17-27 years) in percentage (33%) in comparison withother age groups (20 %) in (27-37 years) , (16%) in (37-47 years) , (14%) in ( 47-57 years) , (10%) in (57-67years) and (7%) in (67-77 years) and in males the results appeared increase the UTI with age , (67-77 years)in percentage (32%) in comparison with other age groups, (3%) in (17-27 years) , (9%) in (27-37 years), (11%) in (37-47 years) , (20%) in ( 47-57 years) , (25%) in (57-67 years) . the results of API20 test thatusing for identification of types of bacteria which cause UTI appeared to 34 isolates of E.coli , 15 isolates ofEnterobacter spp. , 18 isolates of Klebsiella spp. , 11 isolates of Pseudomonas spp. , 13 isolates of S.aureusand 4 isolates of Acinetobacter spp. bacteria .In field of antibiotic sensitivity the results appeared the effectsof third and fourth generations of cephalosporin more than other generations .
Detection of Some Virulence Factors Genes in S. Aureus Isolated From Different Clinical Cases Rawaa M. Mohammed
Journal of Global Pharma Technology Volume 10 Issue 12.
Publisher : Journal of Global Pharma Technology

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Abstract

A total of 60 specimens (urine, burn swabs, and wound swabs) from different clinical cases were collected from patients (in different age groups) who admitted to several health centers in Al-Dewaniya General Teaching Hospital, during a period extending from October 2016 to January 2017. Some virulence factors was investigated for 30 isolates only of MRSA using Single and Multiplex PCR For detection virulence factor genes which both coa gene encoding  production of coagulase, clfA gene encoding for clumping factor, spa gene encoding for protein A, fnbA gene encoding for fibronectin binding proteins, luks gene encoding production of Panton Valentine Leukocidin (PVL), Results showed that all 30 isolates (100%) were possescoa, clfA, spaand fnbA genes, 13 (43.3%) were possesluks gene. Nanoparticle represented by gold and silver were tested in this study to defect their effect on viability of S.aureus. The results indicated that? Particle were more effective to inhibit the grow that of this bacterium (data are not showing in this paper).Keywords: virulence factors, MRSA, PVL, S.aureus, Nanoparticles.
Bacterial Infection Associated with Respiratory Cystic Fibrosis in Al-Hilla City Rawaa M. Mohammed
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 1 (2025): January: OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i1.979

Abstract

Back ground: Autosomal genetic diseases throughout North America are propagated by CF, which is seen in 1 out of 2000 Caucasians. This sickness can in most cases be undetected and is nongendered with a global impact and targets all ages. By the impaired mucocilliary clearance in the trachea and bronchi, and the inability to maintain innate defenses, acute and recurrent bacterial infections, airways obstruction and inflammation and chronic bacterial infections are aggravated. Aim of study: The study aims to isolate and identify bacterial species related to respiratory fibrosis and to do the susceptibility test to those species. Methodology: The study was carried out between January-July 2024, 80 cystic fibrosis patients admitted in Mirjan hospital, 50 came outpatients clinic and 30 found sleeping in the hospital (Eighteen in the respiratory lobbies and 12 in the intensive care unit (ICU)), patient age range between 20-80 years old, and include both gender, 28 females and 52 male.es). By using sterile labeled machines every patient provide sputum that was transported to laboratory directly after collection and stored in 4 C temperatures until culture. All samples of sputum were cultivated for twenty-four hours at 37 degrees Celsius in an incubator utilizing MAConkey and Blood agars. Thereafter, samples were streaked unto several selective and differential media where bacterial colonies matured to reveal their biochemical characteristic after 24hrs of incubation. Results: The current study's results indicated that age has an impact on microbial CF infection. Of the 80 total patients, 9 sample in the age group of 20–30 years had a percentage of 11.25%, while 11 patients in the age group of 31–40 years had a percentage of 13.75%, 16 patients in the age group of 41–50 years had a percentage of 16.25%, and 17 patients in the age group of 51–60 years had a percentage of 21.25%. The current study confirmed that infection rates increase with age. In terms of gender, the study show that males are more likely than females to get infections. with 52 males in the (65%) percentage and 28 females in the (35%), Ten patients are in the 71–80 age range, and twenty patients are in the 61–70 age group. Conclusion: The kind of bacterial strain, the patient's immunological condition, and the usage of contaminated medical equipments are some of the factors that affect transmission. During treatment, all CF patients colonized or infected with the primary pathogens discussed in this article must be kept in a single room to prevent nosocomial dissemination of the microorganism to other patients. Despite the intricacy of the epidemiology of bacterial infections in CF patients, the average lifespan of these individuals continues to increase.
The Impact of the adeE and adeY Genes on the Antibiotic Resistance Mechanisms of Acinetobacter baumannii Rawaa M. Mohammed
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 3 No. 3 (2025): May: OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v3i3.1286

Abstract

Current study identified Acinetobacter baumannii isolates from burn wound samples using biochemical andmolecular techniques focusing on the rpoB gene. All isolates showed variable virulence factors, includingbiofilm formation (100%), gelatinase activity (90%), and mannose-resistant hemagglutination (72%). Antibioticsusceptibility testing revealed high resistance rates to Rifampin (96%), Tetracycline (92%), and third-generation cephalosporins such as Cefotaxime (73%) and Ceftriaxone (81%). Lower resistance rates wereobserved for Levofloxacin (31%) and Gatifloxacin (27%), suggesting potential effectiveness.PCR analysis confirmed the presence of AdeE and AdeY efflux pump genes in several isolates. However,statistical analysis, including Chi-square/Fisher’s tests and t-tests, demonstrated no significant correlationbetween the presence of these genes and antibiotic resistance patterns (p > 0.05). This indicates that whileefflux pump genes are present, their presence alone does not predict antibiotic resistance in the tested isolates.The findings highlight the complex mechanisms of resistance in A. baumannii and the need for continuedsurveillance and molecular diagnostics to guide effective infection control.