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All Journal Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Saad Hasan Mohammed Ali
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Medicine & Pharmacology Public Health

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Molecular Co-Localization of Human Epstein Barr Virus Infection Associated with the Expressed Protein of Cyclin D1 \Cyclin Dependent Kinase 4 in Hodgkin’s Lymphoma Tissues Nouri Jaffar Alalsaidissa; Zahraa Ali Abdullah; Ausama Abed-AlkadumAlajeely; Saad Hasan Mohammed Ali; Shakir H Mohammed Al-Alwany
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14524

Abstract

This retrospective case-control study to assess the impact of Cyclin D1 and CDK4 expression along withEBV infection with Hodgkin’s lymphoma (HL).80 lymph node included in this study; (40) formalin-fixed,paraffin- embedded biopsies from Hodgkin’s lymphoma (HL), and (40) apparently healthy lymph nodes ascontrol group. EBV detection was done by CISH whereas IHC detection system was used to evaluate bothCyclin D1 & CDK4 gene expression.The positive –EBERs CISH reactions detected in 16 / 40 (40 %) of Hodgkin lymphoma tissues while positive- EBERs reactions detected in 3 out of 40 tissues (7.5%) in control lymph nodes tissues. Statistically wasfound highly significant between study groups (P value = < 0.05). Cyclin D1 & CDK4 -IHC were detectedin 40% and 52.5% of HL, respectively. Statistically, significant differences noticed among negative, low andmoderate scoring of tissues (P<0.05).Significant EBV gene expressions along with higher percentage of tissues in which Cyclin D1 & CDK4genes being expressed may be indicator their possible role in the pathogenesis and / or the carcinogenesis ofa subset of our Hodgkin’s lymphoma cases.
Study of Epstein - Barr virus Infection in Relation to the Immunohistochemical Expression of Bcl-2 gene in Tissues of Patients with Adenocarcinoma of the Colon Sarmad M. Zeiny; Saad Hasan Mohammed Ali; Jabbar R. Zangoor; Shakir H .Mohammed Al-Alwany
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14903

Abstract

Background: EBV infection in tissue micro-environment is challenged by the precisely regulated survivaland apoptosis mechanisms. Abnormal bcl-2 proto-oncogene expression in colonic carcinomas allowsaccumulation and propagation of these genetically altered cells.Objective: To analyze the relevant concordance of BCL-2 gene , EBNA1 s and LMP-1-EBV expression inissues from a group of Iraqi patients with colonic adenocarcinomas.Patients and Methods: One hundred (100) tissue biopsies, belonged to (40) patients with colorectalcancers, (40) patients with benign colon tumors, and (20) apparently normal colorectal control tissues,were enrolled in this study. The detection of EBNA1 s and LMP-1-EBV as well as BCL-2 was done byimmunohistochemistry (IHC).Results: The positive-IHC results of EBNA1 detection in 40 tissues with malignant colonic tumors were57.5%, while in 40 colonic benign tumors tissues were 32.5 %. In those apparently healthy colonic controltissues, EBNA1 detection was 20%. The positive results of LMP-1 –EBV -IHC detection where 45 % fromcolonic carcinoma group, while the percentage of benign colonic tumors was 30%(12 out of 40). Lastly, inAHC was15% showed positive signals. Malignant colonic tumors showed 47.5% positive results of Bcl2-IHC detection while benign colonic tumor tissues showed 40% positive bcl-2-IHC reactions and in thecolonic control tissues was 20%.Conclusion: It concluded from the present high rates of EBV infection in colonic adenocarcinoma alongwith the concordance bcl-2 expression that they could played an important role in the development andprogression of both malignant and benign colonic tumors in our group of Iraqi patients.
Assessment of IL-6 , IL-23 and Serum amylase Levels in Patients with Enteric Fevers Khalil Ismail A. Mohammed; Wifaq M. Ali Al-Wattar; Suha A. Al-Fukhar; Saad Hasan Mohammed Ali; Jinan M. Mousa
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15820

Abstract

The study was carried out during the period of March 2019-January 2020 for the detection of Salmonellosis in102 suspected patients with age group ranged from 17 - 69 years, who attended Baghdad teaching hospitals,that had been examined and defined as suspected cases by a specialized physician with the recording ofclinical manifestation. The diagnosis is done by the immunochromatography method, a blood sample wastaken from each patient as well as other 30 healthy control matching in age and gender. The study includedmeasurement of the level of interleukin -6,23 and Amylase activity in sera of patients and healthy control.The result indicated that anti –salmonella IgM positive in 54 cases, anti- salmonella IgG positive in 50cases, and 18 positive cases with both IgM and IgG. The Level of interleukin 6,23 increased significantlywhile the serum amylase activity decreased significantly in patients sera in comparison with healthy control.
Nuclear in Situ Hybridization of High –Risk and Low-Risk Human Papilloma Virus DNA in Tissues from a Group of Iraqi Patients with Sino Nasal and Nasopharyngeal Benign and Malignant Tumors Saad Hasan Mohammed Ali
Journal of Global Pharma Technology Volume 9 Issue 10
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Human papilloma viruses (HPV) are well known viruses that can induce tumor genesis in proliferative epithelial tissues.  Many studies implicated HPV in progression of head and neck benign and malignant tumors by affecting cell cycle proteins expression. Objectives This study was designed to determine the rates of detection high- risk HPV16/18 and low -risk HPV6/11 genotypes in tissues from nasal cavity, par nasal sinuses and nasopharyngeal lesions that include apparently healthy tissue, nasal polyps (NP), sin nasal papillomas (SNP) , nasopharyngeal carcinomas(NPC) and sin nasal carcinomas (SNC).Methods: This study is a retrospective one. Total 125formalin -fixed paraffin embedded tissue blocks including: 35 nasal polyps, 35 sin nasal papilloma S, 29 nasopharyngeal carcinomas 6 sin nasal carcinomas and nasal apparently healthy tissues. After his to pathological confirmation Viral DNA localization performed using chromomeric in situ hybridization (CISH).  Results: In sin nasal papillomas and nasal polypsHPV6/11was detected in 54.3% and 22.9% respectively and in sin nasal and nasopharyngeal carcinomas (SNC+NPC) group was detected in 14.3% where the results have revealed highly significant differences (P<0.01).Percentage of HPV16/18 genotypes in SNC+NPC  group was 31.4 %, in sin nasal papilloma tissues was detected in 25.7% and in nasal polyps tissues was detected in 11.4 %where such results have revealed non-significant differences (P>0.05).Conclusions: The present results point for a possible association between  high on cogenic-risk HPV genotypes with sin nasal papillomas as well as SNC and NPC in the way of their pathogenesis and /or tumor genesis where as a low association was observed between high and low- risk HPV genotypes with nasal polyps lesions.Keywords:  Low- riskHPV-6/11, High- riskHPV-16/18, CISH, Sino nasal and Nasopharyngeal lesions.
Co-Authors Ausama Abed-AlkadumAlajeely Jabbar R. Zangoor Jinan M. Mousa Khalil Ismail A. Mohammed Nouri Jaffar Alalsaidissa Sarmad M. Zeiny Shakir H .Mohammed Al-Alwany Shakir H Mohammed Al-Alwany Suha A. Al-Fukhar Wifaq M. Ali Al-Wattar Zahraa Ali Abdullah