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All Journal Indian Journal of Forensic Medicine & Toxicology
Ahmed Khudhair Al-Hamairy
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Molecular Study of Virulence Factors (Cysteine Protease-1 and Amoebapore-C) for Entamoeba histolytica in Babylon Province Entessar Hatif Abd Al-Kathem Al-Murshidi; Ahmed Khudhair Al-Hamairy; Hadi Meziel Khudhair
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14560

Abstract

A molecular study was conducted for the period from December 2019 to May 2020, which includedexamining (50) positive stool samples by using the direct smear method. that was revealed by the method ofpolymerase chain reaction (PCR) using the gene (18SrRNA), with a percentage of 82%, distributed 87.8%for males and 70.6% for females. Then for determine the virulence factors ( Cysteine proteinase-1) and(Amoebapores-C) by using primers, with (PCR)technique , and then electrophoresis on the agarose gel havebeen done , the results were positive for Cysteine proteinase-1 (APC-1) at a wavelength (212 bp) and theAmoebapore-C (Amo-C) at a wavelength (536 bp). Five random positive samples of the PCR product in thecurrent study were sent to Macrogen in South Korea for the purpose of determining the DNA sequence ofthe amoebapore- C gene present in the Entamoeba histolytica, and it is the first study in Iraq and Babylonprovince that is genetically determined by the NCBI These genotypes of the amoebic holes gene are recordedin the gene bank. The results of the study showed that all the transmitted isolates are identical with NCBIisolates with the USA samples registered with the gene bank with accession number AY956434.2, whichare the isolated samples belonging to the American samples and diagnosed with the number MS30-1043 ofthe USA study by the researcher Bhattacharya et al. (2005) with the current study samples As it was 100%identical. These samples were recorded in the genebank for the first time for this Amoebapore C gene underAccession No. For samples (1, 2, 3, 4, 5) in sequences are MT951203, MT951204, MT951205, MT951206and MT951207, respectively.
Haplotyping of Echinococcus granulosus Isolates From Human, Sheep and Cattles Hydatid Cysts in Some Central Euphrates Provinces, Iraq Nabaa Zahid Sabri Shani Al-Hasnawi; Ahmed Khudhair Al-Hamairy; Huda Jasim M. Altameme
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15504

Abstract

This study included nine samples collected from the infected (liver and lung) divided into three equalsamples (sheep, cattle and humans) collected from some Central Euphrates Provinces for the period from thebeginning August 2019 till February 2020. PCR technique was performed for in detection and genotypingof Echiococcus granulosus hydatid cyst based on mitochondrial Cox1 gene and Nad1 genes in isolates.This technique was carried out according to method described by Nikmanesh et al. (2014). The presentstudy aimed to showed haplotype genetic and the frequency percentage of it in the Central Euphratesregion.the result showed the H15 haplotype was a higher frequency followed it ( H10, H11) and then (H5).The frequency percentage of the haplotype genetic in the current study of the taken samples (8) that theprevalence and frequency of haplotype genetic H15 was 37.5%, followed by the genotype ( H10,H11) at25% for both them , and the lowest was the genotype (H5) at 12.5% The human samples registered 100%for (H15) , the sheep registered 50 % for H5, H10.While cattle samples registered 33.3 % for H10 and 66.6for H11 . Isolation H15 is the essential in the infection to humans as a result of dogs were fed hydatid cystsof cattle origin, and there is no relationship between sheep and humans, while there is a relationship betweensheep and cattle in the Haplotype (H10).
Haplotyping of Echinococcus granulosus Isolates From Human, Sheep and Cattles Hydatid Cysts in Some Central Euphrates Provinces, Iraq Nabaa Zahid Sabri Shani Al-Hasnawi; Ahmed Khudhair Al-Hamairy; Huda Jasim M. Altameme
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15667

Abstract

This study included nine samples collected from the infected (liver and lung) divided into three equal samples (sheep, cattle and humans) collected from some Central Euphrates Provinces for the period from the beginning August 2019 till February 2020. PCR technique was performed for in detection and genotyping of Echiococcus granulosus hydatid cyst based on mitochondrial Cox1 gene and Nad1 genes in isolates. This technique was carried out according to method described by Nikmanesh et al. (2014). The present study aimed to showed haplotype genetic and the frequency percentage of it in the Central Euphrates region. the result showed the H15 haplotype was a higher frequency followed it ( H10, H11) and then (H5). The frequency percentage of the haplotype genetic in the current study of the taken samples (8) that the prevalence and frequency of haplotype genetic H15 was 37.5%, followed by the genotype ( H10,H11) at 25% for both them , and the lowest was the genotype (H5) at 12.5% The human samples registered 100% for (H15) , the sheep registered 50 % for H5, H10.While cattle samples registered 33.3 % for H10 and 66.6 for H11 . Isolation H15 is the essential in the infection to humans as a result of dogs were fed hydatid cysts of cattle origin, and there is no relationship between sheep and humans, while there is a relationship between sheep and cattle in the Haplotype (H10).
Co-Authors Entessar Hatif Abd Al-Kathem Al-Murshidi Hadi Meziel Khudhair Huda Jasim M. Altameme Nabaa Zahid Sabri Shani Al-Hasnawi