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All Journal Indian Journal of Forensic Medicine & Toxicology
Omar A.Mohammed Al Hashimi
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Public Health

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Phenotypic and Molecular Detection of Pyocin from Multidrug-Resistant Pseudomonas aeruginosa Isolated from Various Pathogenic Ahmed Yas Saeed; Dhyaa Fandi Ahmed; Mahmood Khlaf Saleh; Adnan F. Alazzawie; Mostafa Qahtan Mostafa; Fatima Mustafa Mahdii; Nedaa Wasmi Shehab; Omar A.Mohammed Al Hashimi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14577

Abstract

This study included collecting (114) pure isolates of Pseudomonas aeruginosa, distributed over wounds(39) isolates, burns (31) isolates, urinary tract infections (23) isolations, ear infections (12) isolations, andrespiratory infections (9) Isolates. The test for the sensitivity of the bacteria to (14) types of antibioticsshowed a clear variation in their resistance to antibiotics, as all isolates showed 100% resistance toAmpicillin and Novabocin, Nalidixic acid by 93.8%, and Bacitracin by 87.7%, while they had low resistanceto Ciprofloxacin, 21%, Cefotaxime 19.2%, and Chloramphenicol 18.4%. While the results of the phenotypicdetection of pyocin showed the presence of (85 isolates, at a rate of 74.5%), producing Pyocin, while (29isolates, at a rate of 25.4%), it showed its inability to produce Pyosin. As for the molecular detection of thePrtN gene, which is one of the most important genes responsible for the production of Pyocin, It was shownthat (17 isolates, 77.2%) possess this gene, while (5 isolates, at a rate of 22.7%), they did not possess thisgene.
Co-Authors Adnan F. Alazzawie Ahmed Yas Saeed Dhyaa Fandi Ahmed Fatima Mustafa Mahdii Mahmood Khlaf Saleh Mostafa Qahtan Mostafa Nedaa Wasmi Shehab