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All Journal Indian Journal of Forensic Medicine & Toxicology
Altamemy A. K. Aakool
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Public Health

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Molecular Detection of Toxoplasma gondii in Helix pomatia in Wasit Province Iraq Duaa Abdul Khaliq AL-Quraishy; Altamemy A. K. Aakool
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14710

Abstract

DNA of a total of 96 samples of (32) enteric, (32) gastric and (32) pod of Helix pomatia has been obtainedthrough the use of the Genomic DNA Extraction kit and tested by the use of the Real time PCR and specificset of the primers and probe for the detection of the Toxoplasma gondii B1 gene. Six enteric samples and only2 gastric samples have been positive while the others have been negative, that result indicates the presenceof the T. gondii in that snail species, and based upon those results infection can either be acquired from thecontaminated environment or as a result of consuming the grass that has been contaminated with parasiteoocysts. The negative result of pod samples may be due to the failure of filtration the infectious stages ofparasites into the organs that close contacts with environment or inability of the parasites in surviving inthose organs.
Co-Authors Duaa Abdul Khaliq AL-Quraishy