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All Journal Indian Journal of Forensic Medicine & Toxicology
Malinee Pongsavee
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Public Health

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Potassium Sorbate Induces Oxidative Stress and Genotoxicity in Human Lymphocytes Malinee Pongsavee; Rajnikant Mishra
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14795

Abstract

Potassium sorbate, a potassium salt of sorbic acid, has been used as food preservative and antimicrobial agent.Some observations in rat and hamster cells suggest its toxicity. However, observations in human cells arelimiting. Therefore, it has been intended to investigate impacts of potassium sorbate on human lymphocytes.The lymphocytes were cultured and treated with 0.5, 1.0, 1.5 and 2.0 mg/ml of potassium sorbate for 24 and48 h. Evaluations of its impacts were done through the MTT assay, analysis of chromosomal aberrations,formation of micronucleus and activities of superoxide dismutase. The results revealed that potassiumsorbate induced oxidative stress, genotoxicity, damages to chromosome, formation of micronucleus inhuman lymphocytes.
Effects of Trichloroethylene on p53 and Bcl-2 Expression, Chromosome Loss and Catalase Activity Inhibition in Human Malinee Pongsavee
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14796

Abstract

Trichloroethylene (TCE) is a common organic solvent that has been widely used in industrial applications.TCE is highly lipophilic and readily absorbed into the circulation following oral, dermal or inhalationexposure. The purpose of this study is to examine the alterations of p53 and Bcl-2 expression, chromosomeloss and catalase activity inhibition after treatment with TCE. Human lymphocytes were cultured withvarious doses of TCE for 24 h. Quantitative RT-PCR (qRT-PCR) technique, chromosome culture techniqueand catalase activity assay were performed in human lymphocyte cultures. The results of Quantitative RTPCR (qRT-PCR) analysis showed up-regulated expression in the experimental groups comparison with thecontrol groups of p53 gene [0.016 mM/L (1.4-fold) and Bcl-2 gene [0.008 mM/L (1.4-fold) and 0.016mM/L (1.6-fold)]. Loss of chromosome 8 and 22 were observed. Catalase activity was reduced whenTCE concentration increased (p<0.05). It is indicated that TCE effects on p53 and Bcl-2 gene expression,chromosome loss and catalase activity inhibition. These effects may lead to the onset of cancer in the future.
Co-Authors Rajnikant Mishra