Mohammed Hashim AL-Yasiri
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Microbial Infections Associated with Diabetic Foot Ulcer in Nasiriyah City Muna F. Jahad; Mohammed Hashim AL-Yasiri
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14808

Abstract

Diabetic foot ulcers (DFUs) are a major complication of diabetes lead to development of gangrene associatedwith reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity andmortality. This study was aimed to identify the pathogenic microorganisms in diabetic foot ulcers and to testtheir antibiotic susceptibility. A total of 134 patients with diabetic foot ulcers (DFU) attending to Diabeticcenter/ Thi-Qar province, South of Iraq were randomly selected. Samples were cultured for isolating bacteriaspecies and Fungi species. Antibiotic sensitivity testing for bacteria species was done by Kirby Bauer discdiffusion method. Elderly diabetic males were frequently observed to develop foot ulcers higher than female.The most of patients 56 (41.79%) were in the age group 51 to 60 years followed by the 36 (26.86%) patientswere in age group 41 to 50 years. Out of 162 isolates, bacteria species was 116 (71.6%) more than Fungispecies 46 (28.4%). The most isolated bacteria was Proteus mirabilis 25 (15.43%) followed by Enterobactercloacae 22 (13.58%) and both Escherichia coli and Staphylococcus epidermidis 17 (10.49%). Diabetic footinfections are life-threatening. Many pathogens associated with diabetic foot ulcers.
Quantitative and Qualitative Determination of Gliotoxin and Acetaldehyde Toxins in Yeasts Isolated from some Respiratory Patients and Study of Yeast Resistance to some Antibiotics Iman. H. Al Fayyadh; Mohammed Hashim al-Yasiri
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 3 (2022): Indian Journal of Forensic Medicine and Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i3.18282

Abstract

The concentrations (64, 128, 32, 16, 8, 4, 2, 1, 0.5, 0.25 and 0.125) mg/ml were used to calculate the lowestinhibitory concentration of antibiotics for Candida yeast by microdilution method. The antibiotics fluconazoleand itraconazole were used to complete this experiment, which is based on the results of testing the API Candidasystem by demonstrating the resistance of yeasts using several mechanisms, which serve to phenotype eachorganism selected in the framework. In this test, it was found that there is resistance to the antibiotics itraconazoleand fluconazole in the types of yeasts under experiment. The toxin-producing yeasts extracted from yeasts showedthat the toxin-producing yeast species were only 27 out of 30 samples of yeast, despite the growth efficiency of allsamples. While 30 samples of yeast isolated from patients’ sputum, it was found that all samples under the currentstudy contain different amounts of acetaldehyde toxins with different concentrations.